Reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by [D-Trp6]-Luteinizing Hormone Releasing Hormone in hypophysectomized rats

A. Arimura, P. Serafini, S. Talbot, Andrew V Schally

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Adult and immature male rats were hypophysectomized and injected daily with saline or 0.2 or 2 μg of superactive Luteinizing Hormone Releasing Hormone (LHRH) agonist, [D-Trp6]-LHRH subcutaneously for seven days - with, or without, concomitant treatment of 1 IU Human Chorionic Gonadotropin (hCG) or 50 IU Pregnant Mare Serum. The administration of [D-Trp6]-LHRH reduced Luteinizing Hormone/Human Chorionic Gonadotropin receptors in all cases. The magnitude of this reduction was dose-related. As small a dose as 0.2 μg of the peptide resulted in approximately a 72% reduction of the receptors. The results suggest a direct action of [D-Trp6]-LHRH on the testis. It also indicated that reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by the peptide is not necessarily due to the over-stimulation of Luteinizing Hormone (LH) release from the pituitary through a "down regulation" mechanism.

Original languageEnglish
Pages (from-to)687-693
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume90
Issue number3
DOIs
StatePublished - Oct 12 1979
Externally publishedYes

Fingerprint

Testicular Hormones
LH Receptors
Luteinizing Hormone
Gonadotropin-Releasing Hormone
Rats
Peptides
Chorionic Gonadotropin
Testis
Down-Regulation
Serum

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

@article{06de0ebbd98a4e7b9fedd55010232967,
title = "Reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by [D-Trp6]-Luteinizing Hormone Releasing Hormone in hypophysectomized rats",
abstract = "Adult and immature male rats were hypophysectomized and injected daily with saline or 0.2 or 2 μg of superactive Luteinizing Hormone Releasing Hormone (LHRH) agonist, [D-Trp6]-LHRH subcutaneously for seven days - with, or without, concomitant treatment of 1 IU Human Chorionic Gonadotropin (hCG) or 50 IU Pregnant Mare Serum. The administration of [D-Trp6]-LHRH reduced Luteinizing Hormone/Human Chorionic Gonadotropin receptors in all cases. The magnitude of this reduction was dose-related. As small a dose as 0.2 μg of the peptide resulted in approximately a 72{\%} reduction of the receptors. The results suggest a direct action of [D-Trp6]-LHRH on the testis. It also indicated that reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by the peptide is not necessarily due to the over-stimulation of Luteinizing Hormone (LH) release from the pituitary through a {"}down regulation{"} mechanism.",
author = "A. Arimura and P. Serafini and S. Talbot and Schally, {Andrew V}",
year = "1979",
month = "10",
day = "12",
doi = "10.1016/0006-291X(79)91882-5",
language = "English",
volume = "90",
pages = "687--693",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by [D-Trp6]-Luteinizing Hormone Releasing Hormone in hypophysectomized rats

AU - Arimura, A.

AU - Serafini, P.

AU - Talbot, S.

AU - Schally, Andrew V

PY - 1979/10/12

Y1 - 1979/10/12

N2 - Adult and immature male rats were hypophysectomized and injected daily with saline or 0.2 or 2 μg of superactive Luteinizing Hormone Releasing Hormone (LHRH) agonist, [D-Trp6]-LHRH subcutaneously for seven days - with, or without, concomitant treatment of 1 IU Human Chorionic Gonadotropin (hCG) or 50 IU Pregnant Mare Serum. The administration of [D-Trp6]-LHRH reduced Luteinizing Hormone/Human Chorionic Gonadotropin receptors in all cases. The magnitude of this reduction was dose-related. As small a dose as 0.2 μg of the peptide resulted in approximately a 72% reduction of the receptors. The results suggest a direct action of [D-Trp6]-LHRH on the testis. It also indicated that reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by the peptide is not necessarily due to the over-stimulation of Luteinizing Hormone (LH) release from the pituitary through a "down regulation" mechanism.

AB - Adult and immature male rats were hypophysectomized and injected daily with saline or 0.2 or 2 μg of superactive Luteinizing Hormone Releasing Hormone (LHRH) agonist, [D-Trp6]-LHRH subcutaneously for seven days - with, or without, concomitant treatment of 1 IU Human Chorionic Gonadotropin (hCG) or 50 IU Pregnant Mare Serum. The administration of [D-Trp6]-LHRH reduced Luteinizing Hormone/Human Chorionic Gonadotropin receptors in all cases. The magnitude of this reduction was dose-related. As small a dose as 0.2 μg of the peptide resulted in approximately a 72% reduction of the receptors. The results suggest a direct action of [D-Trp6]-LHRH on the testis. It also indicated that reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by the peptide is not necessarily due to the over-stimulation of Luteinizing Hormone (LH) release from the pituitary through a "down regulation" mechanism.

UR - http://www.scopus.com/inward/record.url?scp=0018643942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018643942&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(79)91882-5

DO - 10.1016/0006-291X(79)91882-5

M3 - Article

C2 - 228668

AN - SCOPUS:0018643942

VL - 90

SP - 687

EP - 693

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -