Reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by [D-Trp6]-Luteinizing Hormone Releasing Hormone in hypophysectomized rats

A. Arimura; P. Serafini; S. Talbot; A. V. Schally

doi:10.1016/0006-291X(79)91882-5

Reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by [D-Trp⁶]-Luteinizing Hormone Releasing Hormone in hypophysectomized rats

A. Arimura, P. Serafini, S. Talbot, A. V. Schally

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Adult and immature male rats were hypophysectomized and injected daily with saline or 0.2 or 2 μg of superactive Luteinizing Hormone Releasing Hormone (LHRH) agonist, [D-Trp⁶]-LHRH subcutaneously for seven days - with, or without, concomitant treatment of 1 IU Human Chorionic Gonadotropin (hCG) or 50 IU Pregnant Mare Serum. The administration of [D-Trp⁶]-LHRH reduced Luteinizing Hormone/Human Chorionic Gonadotropin receptors in all cases. The magnitude of this reduction was dose-related. As small a dose as 0.2 μg of the peptide resulted in approximately a 72% reduction of the receptors. The results suggest a direct action of [D-Trp⁶]-LHRH on the testis. It also indicated that reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by the peptide is not necessarily due to the over-stimulation of Luteinizing Hormone (LH) release from the pituitary through a "down regulation" mechanism.

Original language	English (US)
Pages (from-to)	687-693
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	90
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(79)91882-5
State	Published - Oct 12 1979
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by [D-Trp⁶]-Luteinizing Hormone Releasing Hormone in hypophysectomized rats. / Arimura, A.; Serafini, P.; Talbot, S.; Schally, A. V.

In: Biochemical and biophysical research communications, Vol. 90, No. 3, 12.10.1979, p. 687-693.

Research output: Contribution to journal › Article › peer-review

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title = "Reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by [D-Trp6]-Luteinizing Hormone Releasing Hormone in hypophysectomized rats",

abstract = "Adult and immature male rats were hypophysectomized and injected daily with saline or 0.2 or 2 μg of superactive Luteinizing Hormone Releasing Hormone (LHRH) agonist, [D-Trp6]-LHRH subcutaneously for seven days - with, or without, concomitant treatment of 1 IU Human Chorionic Gonadotropin (hCG) or 50 IU Pregnant Mare Serum. The administration of [D-Trp6]-LHRH reduced Luteinizing Hormone/Human Chorionic Gonadotropin receptors in all cases. The magnitude of this reduction was dose-related. As small a dose as 0.2 μg of the peptide resulted in approximately a 72% reduction of the receptors. The results suggest a direct action of [D-Trp6]-LHRH on the testis. It also indicated that reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by the peptide is not necessarily due to the over-stimulation of Luteinizing Hormone (LH) release from the pituitary through a {"}down regulation{"} mechanism.",

author = "A. Arimura and P. Serafini and S. Talbot and Schally, {A. V.}",

note = "Funding Information: Acknowledgements. This work was supported in by USPHS research grants AM 09094, AM 07467, and Veterans Administration.",

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AU - Serafini, P.

AU - Talbot, S.

AU - Schally, A. V.

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N2 - Adult and immature male rats were hypophysectomized and injected daily with saline or 0.2 or 2 μg of superactive Luteinizing Hormone Releasing Hormone (LHRH) agonist, [D-Trp6]-LHRH subcutaneously for seven days - with, or without, concomitant treatment of 1 IU Human Chorionic Gonadotropin (hCG) or 50 IU Pregnant Mare Serum. The administration of [D-Trp6]-LHRH reduced Luteinizing Hormone/Human Chorionic Gonadotropin receptors in all cases. The magnitude of this reduction was dose-related. As small a dose as 0.2 μg of the peptide resulted in approximately a 72% reduction of the receptors. The results suggest a direct action of [D-Trp6]-LHRH on the testis. It also indicated that reduction of testicular Luteinizing Hormone/Human Chorionic Gonadotropin receptors by the peptide is not necessarily due to the over-stimulation of Luteinizing Hormone (LH) release from the pituitary through a "down regulation" mechanism.

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