Abstract
Nitric oxide generation by L-arginine (2 mg/kg/min) infusion during cardiopulmonary bypass (CPB) increases blood flow to all organs and reduces cytokine induced organ damage by reducing the level of marginating neutrophils (Ns). The N-trapping in the oxygenator (OX), arterial filter (AF), cardiotomy reservoir (CR), and N-margination were quantified with indium 111 labeled autologous neutrophils (INN) in nine groups of 40 Yorkshire pigs (30-35 kg). Cardiopulmonary bypass (180 min or 90 min CPB, 90 min reperfusion) was carried out at 2.5-3.5 L/min and at two temperatures (18°C, 28°C). The INN (650-780 μCi) was administered intravenously 15 mins before CPB. All pigs received heparin systemically (activated coagulation time > 400 secs); CPB was instituted with a roller pump, OX (Univox 1.8 m2), AF (0.25 m2), and CR (BCR-3500, Bentley Lab, Irvine, CA). The INN distribution in the device (OX, AF, CR) and organs was imaged with a gamma camera and measured with an ion chamber and a gamma counter. The LA infusion decreased N-trapping, estimated as the percent of injected INN (mean ± standard deviation), in OX from control (2.7 ± 2.02)% to (0.94 ± 0.29)%, and margination in lung from control (48 ± 4)% to minimal levels (23 ± 2)% (p < 0.01). In the CPB reperfusion group, a beneficial effect was observed at LA low dose and toxicity of higher N-margination at 15 mg/kg/min. Neither CPB temperature nor Leumedin affected N-margination significantly.
| Original language | English |
|---|---|
| Journal | ASAIO Journal |
| Volume | 42 |
| Issue number | 5 |
| State | Published - Sep 1 1996 |
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ASJC Scopus subject areas
- Biophysics
- Bioengineering
Cite this
Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model. / Dewanjee, Mrinal K.; Wu, Shu Ming; De, Dibyendu; Nadkarni, Rahool; Gonzalez, Lipton; Dewanjee, Sumit; Novak, Stana; Perryman, Richard A.; Serafini, Aldo N; Sfakianakis, George N; Duncan, Robert C.; Dalton Dietrich, W.; Ganz, William I.; Hsu, Li Chien.
In: ASAIO Journal, Vol. 42, No. 5, 01.09.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model
AU - Dewanjee, Mrinal K.
AU - Wu, Shu Ming
AU - De, Dibyendu
AU - Nadkarni, Rahool
AU - Gonzalez, Lipton
AU - Dewanjee, Sumit
AU - Novak, Stana
AU - Perryman, Richard A.
AU - Serafini, Aldo N
AU - Sfakianakis, George N
AU - Duncan, Robert C.
AU - Dalton Dietrich, W.
AU - Ganz, William I.
AU - Hsu, Li Chien
PY - 1996/9/1
Y1 - 1996/9/1
N2 - Nitric oxide generation by L-arginine (2 mg/kg/min) infusion during cardiopulmonary bypass (CPB) increases blood flow to all organs and reduces cytokine induced organ damage by reducing the level of marginating neutrophils (Ns). The N-trapping in the oxygenator (OX), arterial filter (AF), cardiotomy reservoir (CR), and N-margination were quantified with indium 111 labeled autologous neutrophils (INN) in nine groups of 40 Yorkshire pigs (30-35 kg). Cardiopulmonary bypass (180 min or 90 min CPB, 90 min reperfusion) was carried out at 2.5-3.5 L/min and at two temperatures (18°C, 28°C). The INN (650-780 μCi) was administered intravenously 15 mins before CPB. All pigs received heparin systemically (activated coagulation time > 400 secs); CPB was instituted with a roller pump, OX (Univox 1.8 m2), AF (0.25 m2), and CR (BCR-3500, Bentley Lab, Irvine, CA). The INN distribution in the device (OX, AF, CR) and organs was imaged with a gamma camera and measured with an ion chamber and a gamma counter. The LA infusion decreased N-trapping, estimated as the percent of injected INN (mean ± standard deviation), in OX from control (2.7 ± 2.02)% to (0.94 ± 0.29)%, and margination in lung from control (48 ± 4)% to minimal levels (23 ± 2)% (p < 0.01). In the CPB reperfusion group, a beneficial effect was observed at LA low dose and toxicity of higher N-margination at 15 mg/kg/min. Neither CPB temperature nor Leumedin affected N-margination significantly.
AB - Nitric oxide generation by L-arginine (2 mg/kg/min) infusion during cardiopulmonary bypass (CPB) increases blood flow to all organs and reduces cytokine induced organ damage by reducing the level of marginating neutrophils (Ns). The N-trapping in the oxygenator (OX), arterial filter (AF), cardiotomy reservoir (CR), and N-margination were quantified with indium 111 labeled autologous neutrophils (INN) in nine groups of 40 Yorkshire pigs (30-35 kg). Cardiopulmonary bypass (180 min or 90 min CPB, 90 min reperfusion) was carried out at 2.5-3.5 L/min and at two temperatures (18°C, 28°C). The INN (650-780 μCi) was administered intravenously 15 mins before CPB. All pigs received heparin systemically (activated coagulation time > 400 secs); CPB was instituted with a roller pump, OX (Univox 1.8 m2), AF (0.25 m2), and CR (BCR-3500, Bentley Lab, Irvine, CA). The INN distribution in the device (OX, AF, CR) and organs was imaged with a gamma camera and measured with an ion chamber and a gamma counter. The LA infusion decreased N-trapping, estimated as the percent of injected INN (mean ± standard deviation), in OX from control (2.7 ± 2.02)% to (0.94 ± 0.29)%, and margination in lung from control (48 ± 4)% to minimal levels (23 ± 2)% (p < 0.01). In the CPB reperfusion group, a beneficial effect was observed at LA low dose and toxicity of higher N-margination at 15 mg/kg/min. Neither CPB temperature nor Leumedin affected N-margination significantly.
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M3 - Article
VL - 42
JO - ASAIO Journal
JF - ASAIO Journal
SN - 1058-2916
IS - 5
ER -