Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model

Mrinal K. Dewanjee, Shu Ming Wu, Dibyendu De, Rahool Nadkarni, Lipton Gonzalez, Sumit Dewanjee, Stana Novak, Richard A. Perryman, Aldo N. Serafini, George N. Sfakianakis, Robert C. Duncan, W. Dalton Dietrich, William I. Ganz, Li Chien Hsu

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Abstract

Nitric oxide generation by L-arginine (2 mg/kg/min) infusion during cardiopulmonary bypass (CPB) increases blood flow to all organs and reduces cytokine induced organ damage by reducing the level of marginating neutrophils (Ns). The N-trapping in the oxygenator (OX), arterial filter (AF), cardiotomy reservoir (CR), and N-margination were quantified with indium 111 labeled autologous neutrophils (INN) in nine groups of 40 Yorkshire pigs (30-35 kg). Cardiopulmonary bypass (180 min or 90 min CPB, 90 min reperfusion) was carried out at 2.5-3.5 L/min and at two temperatures (18°C, 28°C). The INN (650-780 μCi) was administered intravenously 15 mins before CPB. All pigs received heparin systemically (activated coagulation time > 400 secs); CPB was instituted with a roller pump, OX (Univox 1.8 m2), AF (0.25 m2), and CR (BCR-3500, Bentley Lab, Irvine, CA). The INN distribution in the device (OX, AF, CR) and organs was imaged with a gamma camera and measured with an ion chamber and a gamma counter. The LA infusion decreased N-trapping, estimated as the percent of injected INN (mean ± standard deviation), in OX from control (2.7 ± 2.02)% to (0.94 ± 0.29)%, and margination in lung from control (48 ± 4)% to minimal levels (23 ± 2)% (p < 0.01). In the CPB reperfusion group, a beneficial effect was observed at LA low dose and toxicity of higher N-margination at 15 mg/kg/min. Neither CPB temperature nor Leumedin affected N-margination significantly.

Original languageEnglish (US)
Pages (from-to)M661-M666
JournalASAIO Journal
Volume42
Issue number5
StatePublished - Sep 1 1996

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ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Dewanjee, M. K., Wu, S. M., De, D., Nadkarni, R., Gonzalez, L., Dewanjee, S., Novak, S., Perryman, R. A., Serafini, A. N., Sfakianakis, G. N., Duncan, R. C., Dietrich, W. D., Ganz, W. I., & Hsu, L. C. (1996). Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model. ASAIO Journal, 42(5), M661-M666.