Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model

Mrinal K. Dewanjee, Shu Ming Wu, Dibyendu De, Rahool Nadkarni, Lipton Gonzalez, Sumit Dewanjee, Stana Novak, Richard A. Perryman, Aldo N Serafini, George N Sfakianakis, Robert C. Duncan, W. Dalton Dietrich, William I. Ganz, Li Chien Hsu

Research output: Contribution to journalArticle

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Abstract

Nitric oxide generation by L-arginine (2 mg/kg/min) infusion during cardiopulmonary bypass (CPB) increases blood flow to all organs and reduces cytokine induced organ damage by reducing the level of marginating neutrophils (Ns). The N-trapping in the oxygenator (OX), arterial filter (AF), cardiotomy reservoir (CR), and N-margination were quantified with indium 111 labeled autologous neutrophils (INN) in nine groups of 40 Yorkshire pigs (30-35 kg). Cardiopulmonary bypass (180 min or 90 min CPB, 90 min reperfusion) was carried out at 2.5-3.5 L/min and at two temperatures (18°C, 28°C). The INN (650-780 μCi) was administered intravenously 15 mins before CPB. All pigs received heparin systemically (activated coagulation time > 400 secs); CPB was instituted with a roller pump, OX (Univox 1.8 m2), AF (0.25 m2), and CR (BCR-3500, Bentley Lab, Irvine, CA). The INN distribution in the device (OX, AF, CR) and organs was imaged with a gamma camera and measured with an ion chamber and a gamma counter. The LA infusion decreased N-trapping, estimated as the percent of injected INN (mean ± standard deviation), in OX from control (2.7 ± 2.02)% to (0.94 ± 0.29)%, and margination in lung from control (48 ± 4)% to minimal levels (23 ± 2)% (p < 0.01). In the CPB reperfusion group, a beneficial effect was observed at LA low dose and toxicity of higher N-margination at 15 mg/kg/min. Neither CPB temperature nor Leumedin affected N-margination significantly.

Original languageEnglish
JournalASAIO Journal
Volume42
Issue number5
StatePublished - Sep 1 1996

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Oxygenators
Arginine
Cardiopulmonary Bypass
Neutrophils
Swine
Indium
Ionization chambers
Nitric oxide
Reperfusion
Coagulation
Toxicity
Heparin
Nitric Oxide
Blood
Cameras
Pumps
Gamma Cameras
Cytokines
Temperature
Ions

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Dewanjee, M. K., Wu, S. M., De, D., Nadkarni, R., Gonzalez, L., Dewanjee, S., ... Hsu, L. C. (1996). Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model. ASAIO Journal, 42(5).

Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model. / Dewanjee, Mrinal K.; Wu, Shu Ming; De, Dibyendu; Nadkarni, Rahool; Gonzalez, Lipton; Dewanjee, Sumit; Novak, Stana; Perryman, Richard A.; Serafini, Aldo N; Sfakianakis, George N; Duncan, Robert C.; Dalton Dietrich, W.; Ganz, William I.; Hsu, Li Chien.

In: ASAIO Journal, Vol. 42, No. 5, 01.09.1996.

Research output: Contribution to journalArticle

Dewanjee, MK, Wu, SM, De, D, Nadkarni, R, Gonzalez, L, Dewanjee, S, Novak, S, Perryman, RA, Serafini, AN, Sfakianakis, GN, Duncan, RC, Dalton Dietrich, W, Ganz, WI & Hsu, LC 1996, 'Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model', ASAIO Journal, vol. 42, no. 5.
Dewanjee MK, Wu SM, De D, Nadkarni R, Gonzalez L, Dewanjee S et al. Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model. ASAIO Journal. 1996 Sep 1;42(5).
Dewanjee, Mrinal K. ; Wu, Shu Ming ; De, Dibyendu ; Nadkarni, Rahool ; Gonzalez, Lipton ; Dewanjee, Sumit ; Novak, Stana ; Perryman, Richard A. ; Serafini, Aldo N ; Sfakianakis, George N ; Duncan, Robert C. ; Dalton Dietrich, W. ; Ganz, William I. ; Hsu, Li Chien. / Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model. In: ASAIO Journal. 1996 ; Vol. 42, No. 5.
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abstract = "Nitric oxide generation by L-arginine (2 mg/kg/min) infusion during cardiopulmonary bypass (CPB) increases blood flow to all organs and reduces cytokine induced organ damage by reducing the level of marginating neutrophils (Ns). The N-trapping in the oxygenator (OX), arterial filter (AF), cardiotomy reservoir (CR), and N-margination were quantified with indium 111 labeled autologous neutrophils (INN) in nine groups of 40 Yorkshire pigs (30-35 kg). Cardiopulmonary bypass (180 min or 90 min CPB, 90 min reperfusion) was carried out at 2.5-3.5 L/min and at two temperatures (18°C, 28°C). The INN (650-780 μCi) was administered intravenously 15 mins before CPB. All pigs received heparin systemically (activated coagulation time > 400 secs); CPB was instituted with a roller pump, OX (Univox 1.8 m2), AF (0.25 m2), and CR (BCR-3500, Bentley Lab, Irvine, CA). The INN distribution in the device (OX, AF, CR) and organs was imaged with a gamma camera and measured with an ion chamber and a gamma counter. The LA infusion decreased N-trapping, estimated as the percent of injected INN (mean ± standard deviation), in OX from control (2.7 ± 2.02){\%} to (0.94 ± 0.29){\%}, and margination in lung from control (48 ± 4){\%} to minimal levels (23 ± 2){\%} (p < 0.01). In the CPB reperfusion group, a beneficial effect was observed at LA low dose and toxicity of higher N-margination at 15 mg/kg/min. Neither CPB temperature nor Leumedin affected N-margination significantly.",
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AU - Wu, Shu Ming

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AU - Gonzalez, Lipton

AU - Dewanjee, Sumit

AU - Novak, Stana

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AU - Sfakianakis, George N

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AU - Hsu, Li Chien

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N2 - Nitric oxide generation by L-arginine (2 mg/kg/min) infusion during cardiopulmonary bypass (CPB) increases blood flow to all organs and reduces cytokine induced organ damage by reducing the level of marginating neutrophils (Ns). The N-trapping in the oxygenator (OX), arterial filter (AF), cardiotomy reservoir (CR), and N-margination were quantified with indium 111 labeled autologous neutrophils (INN) in nine groups of 40 Yorkshire pigs (30-35 kg). Cardiopulmonary bypass (180 min or 90 min CPB, 90 min reperfusion) was carried out at 2.5-3.5 L/min and at two temperatures (18°C, 28°C). The INN (650-780 μCi) was administered intravenously 15 mins before CPB. All pigs received heparin systemically (activated coagulation time > 400 secs); CPB was instituted with a roller pump, OX (Univox 1.8 m2), AF (0.25 m2), and CR (BCR-3500, Bentley Lab, Irvine, CA). The INN distribution in the device (OX, AF, CR) and organs was imaged with a gamma camera and measured with an ion chamber and a gamma counter. The LA infusion decreased N-trapping, estimated as the percent of injected INN (mean ± standard deviation), in OX from control (2.7 ± 2.02)% to (0.94 ± 0.29)%, and margination in lung from control (48 ± 4)% to minimal levels (23 ± 2)% (p < 0.01). In the CPB reperfusion group, a beneficial effect was observed at LA low dose and toxicity of higher N-margination at 15 mg/kg/min. Neither CPB temperature nor Leumedin affected N-margination significantly.

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