TY - JOUR
T1 - Reduction of fibrous adhesion formation by a copolymer possessing an affinity for anionic surfaces
AU - Elbert, Donald L.
AU - Hubbell, Jeffrey A.
PY - 1998/10
Y1 - 1998/10
N2 - Postsurgical adhesions represent a common complication following a variety of surgical procedures. We sought to develop and evaluate a water- soluble polymer that could self-assemble onto tissue surfaces, forming a barrier on the surface. A copolymer was synthesized so as to contain two components: one component adsorbed to the tissue surface, and the other created a steric barrier, thereby preventing cell interactions with the tissue surface, and perhaps altering the wound-healing response that leads to the formation of fibrous adhesions. The component selected for tissue binding was a water-soluble polycation, poly-L-lysine, which can bind to negative sites on glycoproteins, proteoglycans, and cells; and the component selected for steric stabilization was polyethylene glycol, a nonionic polymer that interacts poorly with proteins. Efficacy of lavage with an aqueous solution of the copolymer for the prevention of postsurgical abdominopelvic adhesions was assessed following a standard electrocautery injury of the uterine horns of rats. The copolymer resulted in an 88% reduction in the extent of adhesions that formed. In vitro studies designed to investigate the mechanism of this efficacy indicated that the copolymer may both hinder cell-tissue adhesive interactions and alter the process of fibrin formation.
AB - Postsurgical adhesions represent a common complication following a variety of surgical procedures. We sought to develop and evaluate a water- soluble polymer that could self-assemble onto tissue surfaces, forming a barrier on the surface. A copolymer was synthesized so as to contain two components: one component adsorbed to the tissue surface, and the other created a steric barrier, thereby preventing cell interactions with the tissue surface, and perhaps altering the wound-healing response that leads to the formation of fibrous adhesions. The component selected for tissue binding was a water-soluble polycation, poly-L-lysine, which can bind to negative sites on glycoproteins, proteoglycans, and cells; and the component selected for steric stabilization was polyethylene glycol, a nonionic polymer that interacts poorly with proteins. Efficacy of lavage with an aqueous solution of the copolymer for the prevention of postsurgical abdominopelvic adhesions was assessed following a standard electrocautery injury of the uterine horns of rats. The copolymer resulted in an 88% reduction in the extent of adhesions that formed. In vitro studies designed to investigate the mechanism of this efficacy indicated that the copolymer may both hinder cell-tissue adhesive interactions and alter the process of fibrin formation.
KW - Cell culture
KW - Comb copolymer
KW - Polyethylene glycol
KW - Polylysine
KW - Postsurgical adhesions
UR - http://www.scopus.com/inward/record.url?scp=0032188875&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032188875&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4636(199810)42:1<55::AID-JBM8>3.0.CO;2-N
DO - 10.1002/(SICI)1097-4636(199810)42:1<55::AID-JBM8>3.0.CO;2-N
M3 - Article
C2 - 9740007
AN - SCOPUS:0032188875
VL - 42
SP - 55
EP - 65
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
SN - 0021-9304
IS - 1
ER -