Abstract
The CD1d-binding glycolipid α-galactosylceramide exerts potent adjuvant effects on T-dependent humoral immunity. The mechanism is driven by cognate interaction between CD1d-expressing B cells and TCR-expressing type I CD1d-restricted NKT cells. Thus, far positive effects of alpha-galactosylceramide have been observed on initial and sustained antibody titers as well as B-cell memory. Following vaccination, each of these features is desirable, but good B-cell memory is of paramount importance for long-lived immunity. We therefore tested the hypothesis that CD1d expression in vivo differentially affects initial antibody titers versus B-cell memory responses. CD1d+/+ and CD1d+/- mice were generated and immunized with antigen plus CD1d ligand before analysis of cytokine expression, CD40L expression, initial and longer term antibody responses and B-cell memory. As compared with CD1d+/+ controls, CD1d+/- mice had equivalent numbers of total NKT cells, lower cytokine production, fewer CD40L-expressing NKT cells, lower initial antibody responses, similar long-term antibody responses and higher B-cell memory. Our data indicate that weak CD1d antigen presentation may facilitate good B-cell memory without compromising antibody responses. This work may impact vaccine design since over-stimulation of NKT cells at the time of vaccination may not lead to optimal B-cell memory.
Original language | English (US) |
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Pages (from-to) | 251-260 |
Number of pages | 10 |
Journal | International Immunology |
Volume | 23 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2011 |
Externally published | Yes |
Keywords
- Antibody
- B lymphocyte
- CD1d
- Memory
- NKT cell
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology