Reduction of bleomycin-induced acute DNA injury in the rat lung by the 21-aminosteroid, U-74389G

Joseph J. Dallessio, Gwenn E McLaughlin, Lee Frank

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: To determine whether pretreatment with a 21-aminosteroid, U- 74389G, can prevent subsequent DNA injury in bleomycin-exposed lungs. Subjects: Thirty-six adult male Sprague-Dawley rats. Design: Controlled animal laboratory investigation of DNA injury in vivo. Interventions: Animals were treated with 21-aminosteroid (10 mg/kg) or vehicle and subsequently received intratracheal instillation of bleomycin (1.75 U) or normal saline. Measurements and Main Results: Twenty-four hours after bleomycin exposure, the 21-aminosteroid-treated animals had decreased evidence of DNA injury, expressed as percentage of DNA fragmentation normalized to the control group (113.5 ± 6 [SEM] vs. 132 ± 3.9%, p ≤ .05), and activity of the DNA repair enzyme poly ADP-ribose synthetase (3.4 ± 0.2 vs. 5.0 ± 0.9 pmol nicotinamide adenine dinucleotide/min/mg protein, p ≤ .05). Only bleomycin- exposed (+ vehicle) animals demonstrated significant evidence of increased DNA injury vs. the intratracheal saline-exposed control groups. Conclusions: The 21-aminosteroid pretreatment decreases subsequent pulmonary DNA injury induced by bleomycin exposure. This finding is likely due to the 21- aminosteroid's iron-chelating and cell-permeating abilities, and suggests that these agents may be effective in other diseases where iron-dependent free radical reactions occur.

Original languageEnglish
Pages (from-to)652-656
Number of pages5
JournalCritical Care Medicine
Volume25
Issue number4
StatePublished - Apr 1 1997

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Bleomycin
DNA Damage
Lung
Iron
DNA Repair Enzymes
Poly Adenosine Diphosphate Ribose
Control Groups
Laboratory Animals
Lung Injury
DNA Fragmentation
Ligases
NAD
Free Radicals
Sprague Dawley Rats
U 74389F
Proteins

Keywords

  • bleomycin
  • DNA fragmentation
  • iron chelation
  • lipid peroxidation
  • lung injury
  • oxygen free radicals
  • poly(ADP-ribose) synthetase

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Reduction of bleomycin-induced acute DNA injury in the rat lung by the 21-aminosteroid, U-74389G. / Dallessio, Joseph J.; McLaughlin, Gwenn E; Frank, Lee.

In: Critical Care Medicine, Vol. 25, No. 4, 01.04.1997, p. 652-656.

Research output: Contribution to journalArticle

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abstract = "Objective: To determine whether pretreatment with a 21-aminosteroid, U- 74389G, can prevent subsequent DNA injury in bleomycin-exposed lungs. Subjects: Thirty-six adult male Sprague-Dawley rats. Design: Controlled animal laboratory investigation of DNA injury in vivo. Interventions: Animals were treated with 21-aminosteroid (10 mg/kg) or vehicle and subsequently received intratracheal instillation of bleomycin (1.75 U) or normal saline. Measurements and Main Results: Twenty-four hours after bleomycin exposure, the 21-aminosteroid-treated animals had decreased evidence of DNA injury, expressed as percentage of DNA fragmentation normalized to the control group (113.5 ± 6 [SEM] vs. 132 ± 3.9{\%}, p ≤ .05), and activity of the DNA repair enzyme poly ADP-ribose synthetase (3.4 ± 0.2 vs. 5.0 ± 0.9 pmol nicotinamide adenine dinucleotide/min/mg protein, p ≤ .05). Only bleomycin- exposed (+ vehicle) animals demonstrated significant evidence of increased DNA injury vs. the intratracheal saline-exposed control groups. Conclusions: The 21-aminosteroid pretreatment decreases subsequent pulmonary DNA injury induced by bleomycin exposure. This finding is likely due to the 21- aminosteroid's iron-chelating and cell-permeating abilities, and suggests that these agents may be effective in other diseases where iron-dependent free radical reactions occur.",
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N2 - Objective: To determine whether pretreatment with a 21-aminosteroid, U- 74389G, can prevent subsequent DNA injury in bleomycin-exposed lungs. Subjects: Thirty-six adult male Sprague-Dawley rats. Design: Controlled animal laboratory investigation of DNA injury in vivo. Interventions: Animals were treated with 21-aminosteroid (10 mg/kg) or vehicle and subsequently received intratracheal instillation of bleomycin (1.75 U) or normal saline. Measurements and Main Results: Twenty-four hours after bleomycin exposure, the 21-aminosteroid-treated animals had decreased evidence of DNA injury, expressed as percentage of DNA fragmentation normalized to the control group (113.5 ± 6 [SEM] vs. 132 ± 3.9%, p ≤ .05), and activity of the DNA repair enzyme poly ADP-ribose synthetase (3.4 ± 0.2 vs. 5.0 ± 0.9 pmol nicotinamide adenine dinucleotide/min/mg protein, p ≤ .05). Only bleomycin- exposed (+ vehicle) animals demonstrated significant evidence of increased DNA injury vs. the intratracheal saline-exposed control groups. Conclusions: The 21-aminosteroid pretreatment decreases subsequent pulmonary DNA injury induced by bleomycin exposure. This finding is likely due to the 21- aminosteroid's iron-chelating and cell-permeating abilities, and suggests that these agents may be effective in other diseases where iron-dependent free radical reactions occur.

AB - Objective: To determine whether pretreatment with a 21-aminosteroid, U- 74389G, can prevent subsequent DNA injury in bleomycin-exposed lungs. Subjects: Thirty-six adult male Sprague-Dawley rats. Design: Controlled animal laboratory investigation of DNA injury in vivo. Interventions: Animals were treated with 21-aminosteroid (10 mg/kg) or vehicle and subsequently received intratracheal instillation of bleomycin (1.75 U) or normal saline. Measurements and Main Results: Twenty-four hours after bleomycin exposure, the 21-aminosteroid-treated animals had decreased evidence of DNA injury, expressed as percentage of DNA fragmentation normalized to the control group (113.5 ± 6 [SEM] vs. 132 ± 3.9%, p ≤ .05), and activity of the DNA repair enzyme poly ADP-ribose synthetase (3.4 ± 0.2 vs. 5.0 ± 0.9 pmol nicotinamide adenine dinucleotide/min/mg protein, p ≤ .05). Only bleomycin- exposed (+ vehicle) animals demonstrated significant evidence of increased DNA injury vs. the intratracheal saline-exposed control groups. Conclusions: The 21-aminosteroid pretreatment decreases subsequent pulmonary DNA injury induced by bleomycin exposure. This finding is likely due to the 21- aminosteroid's iron-chelating and cell-permeating abilities, and suggests that these agents may be effective in other diseases where iron-dependent free radical reactions occur.

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