Abstract
Patients with HIV infection manifest increased T lymphocyte apoptosis. This study investigated the influence of antiretroviral therapy (ART) upon lymphocyte apoptosis in 23 HIV-infected adults naive to protease inhibitors. Patients were enrolled in a treatment trial consisting of Nelfinavir (NFV), d4T, or NFV + d4T for 24 weeks, followed by triple therapy (NFV + reverse transcriptase inhibitors) for an additional 24 weeks. Spontaneous T cell apoptosis in cultured PBMC decreased by 23.67 ± 18.2% (P < 0.006) at 48 weeks and plasma HIV RNA decreased by 1.79 ± 0.59 log10 RNA copies/ml (P < 0.001). The absolute decrease and slope of T cell apoptosis correlated with plasma virus load and with activated CD8 T cells and was inversely correlated with CD4 T cells. We conclude that reduction in chronic antigenic stimulation and the absence of cellular signals elicited by viral products contribute to the rescue of T lymphocytes from apoptosis, which facilitates immunologic recovery in ART-treated patients.
| Original language | English |
|---|---|
| Pages (from-to) | 24-33 |
| Number of pages | 10 |
| Journal | Clinical Immunology |
| Volume | 93 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1 1999 |
| Externally published | Yes |
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Keywords
- Apoptosis
- FACS
- HIV/AIDS
- Human
- T-lymphocytes
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
- Pathology and Forensic Medicine
Cite this
Reduction in T cell apoptosis in patients with HIV disease following antiretroviral therapy. / Chavan, Surendra J.; Tamma, Seetha L.; Kaplan, Mark; Gersten, Merril; Pahwa, Savita G.
In: Clinical Immunology, Vol. 93, No. 1, 01.10.1999, p. 24-33.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduction in T cell apoptosis in patients with HIV disease following antiretroviral therapy
AU - Chavan, Surendra J.
AU - Tamma, Seetha L.
AU - Kaplan, Mark
AU - Gersten, Merril
AU - Pahwa, Savita G
PY - 1999/10/1
Y1 - 1999/10/1
N2 - Patients with HIV infection manifest increased T lymphocyte apoptosis. This study investigated the influence of antiretroviral therapy (ART) upon lymphocyte apoptosis in 23 HIV-infected adults naive to protease inhibitors. Patients were enrolled in a treatment trial consisting of Nelfinavir (NFV), d4T, or NFV + d4T for 24 weeks, followed by triple therapy (NFV + reverse transcriptase inhibitors) for an additional 24 weeks. Spontaneous T cell apoptosis in cultured PBMC decreased by 23.67 ± 18.2% (P < 0.006) at 48 weeks and plasma HIV RNA decreased by 1.79 ± 0.59 log10 RNA copies/ml (P < 0.001). The absolute decrease and slope of T cell apoptosis correlated with plasma virus load and with activated CD8 T cells and was inversely correlated with CD4 T cells. We conclude that reduction in chronic antigenic stimulation and the absence of cellular signals elicited by viral products contribute to the rescue of T lymphocytes from apoptosis, which facilitates immunologic recovery in ART-treated patients.
AB - Patients with HIV infection manifest increased T lymphocyte apoptosis. This study investigated the influence of antiretroviral therapy (ART) upon lymphocyte apoptosis in 23 HIV-infected adults naive to protease inhibitors. Patients were enrolled in a treatment trial consisting of Nelfinavir (NFV), d4T, or NFV + d4T for 24 weeks, followed by triple therapy (NFV + reverse transcriptase inhibitors) for an additional 24 weeks. Spontaneous T cell apoptosis in cultured PBMC decreased by 23.67 ± 18.2% (P < 0.006) at 48 weeks and plasma HIV RNA decreased by 1.79 ± 0.59 log10 RNA copies/ml (P < 0.001). The absolute decrease and slope of T cell apoptosis correlated with plasma virus load and with activated CD8 T cells and was inversely correlated with CD4 T cells. We conclude that reduction in chronic antigenic stimulation and the absence of cellular signals elicited by viral products contribute to the rescue of T lymphocytes from apoptosis, which facilitates immunologic recovery in ART-treated patients.
KW - Apoptosis
KW - FACS
KW - HIV/AIDS
KW - Human
KW - T-lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=0032869708&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032869708&partnerID=8YFLogxK
U2 - 10.1006/clim.1999.4770
DO - 10.1006/clim.1999.4770
M3 - Article
C2 - 10497008
AN - SCOPUS:0032869708
VL - 93
SP - 24
EP - 33
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 1
ER -