We studied protein synthesis in cervical anterior horn neurons in the "wobbler" mouse by measuring l-[4,5-3H]leucine incorporation using a light microscopic autoradiographic technique. Cytoplasmic incorporation of the isotope was significantly reduced not only in vacuolated nerve cells but also in those which were microscopically normal suggesting that impaired protein synthetic activity precedes the earliest morphologic changes seen with the light microscope. Isotope incorporation was reduced in small as well as large neurons suggesting that the disease process is not confined to α-motoneurons.
ASJC Scopus subject areas
- Developmental Neuroscience