Abstract
The capacity to class switch the IgH chain is critical to the effectiveness of humoral immune responses. We show that in vitro-stimulated splenic B cells from senescent mice are deficient in production of multiple class switch isotypes (IgG1, G2a, G3, and E), class switch recombination (CSR), and induction of the E2A-encoded transcription factor E47. E47 has previously been shown to be required for CSR, at least in part via expression of the activation-induced cytidine deaminase. Our studies show that impaired induction of E47, and subsequently activation-induced cytidine deaminase, contribute to poor CSR and production of secondary isotypes in senescence.
| Original language | English |
|---|---|
| Pages (from-to) | 2155-2162 |
| Number of pages | 8 |
| Journal | Journal of Immunology |
| Volume | 172 |
| Issue number | 4 |
| State | Published - Feb 15 2004 |
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ASJC Scopus subject areas
- Immunology
Cite this
Reduced Ig Class Switch in Aged Mice Correlates with Decreased E47 and Activation-Induced Cytidine Deaminase. / Frasca, Daniela; Van der Put, Elaine; Riley, Richard L; Blomberg, Bonnie B.
In: Journal of Immunology, Vol. 172, No. 4, 15.02.2004, p. 2155-2162.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduced Ig Class Switch in Aged Mice Correlates with Decreased E47 and Activation-Induced Cytidine Deaminase
AU - Frasca, Daniela
AU - Van der Put, Elaine
AU - Riley, Richard L
AU - Blomberg, Bonnie B
PY - 2004/2/15
Y1 - 2004/2/15
N2 - The capacity to class switch the IgH chain is critical to the effectiveness of humoral immune responses. We show that in vitro-stimulated splenic B cells from senescent mice are deficient in production of multiple class switch isotypes (IgG1, G2a, G3, and E), class switch recombination (CSR), and induction of the E2A-encoded transcription factor E47. E47 has previously been shown to be required for CSR, at least in part via expression of the activation-induced cytidine deaminase. Our studies show that impaired induction of E47, and subsequently activation-induced cytidine deaminase, contribute to poor CSR and production of secondary isotypes in senescence.
AB - The capacity to class switch the IgH chain is critical to the effectiveness of humoral immune responses. We show that in vitro-stimulated splenic B cells from senescent mice are deficient in production of multiple class switch isotypes (IgG1, G2a, G3, and E), class switch recombination (CSR), and induction of the E2A-encoded transcription factor E47. E47 has previously been shown to be required for CSR, at least in part via expression of the activation-induced cytidine deaminase. Our studies show that impaired induction of E47, and subsequently activation-induced cytidine deaminase, contribute to poor CSR and production of secondary isotypes in senescence.
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UR - http://www.scopus.com/inward/citedby.url?scp=0842321787&partnerID=8YFLogxK
M3 - Article
C2 - 14764681
AN - SCOPUS:0842321787
VL - 172
SP - 2155
EP - 2162
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 4
ER -