Reduced Ig Class Switch in Aged Mice Correlates with Decreased E47 and Activation-Induced Cytidine Deaminase

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The capacity to class switch the IgH chain is critical to the effectiveness of humoral immune responses. We show that in vitro-stimulated splenic B cells from senescent mice are deficient in production of multiple class switch isotypes (IgG1, G2a, G3, and E), class switch recombination (CSR), and induction of the E2A-encoded transcription factor E47. E47 has previously been shown to be required for CSR, at least in part via expression of the activation-induced cytidine deaminase. Our studies show that impaired induction of E47, and subsequently activation-induced cytidine deaminase, contribute to poor CSR and production of secondary isotypes in senescence.

Original languageEnglish
Pages (from-to)2155-2162
Number of pages8
JournalJournal of Immunology
Volume172
Issue number4
StatePublished - Feb 15 2004

Fingerprint

Genetic Recombination
Humoral Immunity
B-Lymphocytes
Transcription Factors
Immunoglobulin G
AICDA (activation-induced cytidine deaminase)
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Reduced Ig Class Switch in Aged Mice Correlates with Decreased E47 and Activation-Induced Cytidine Deaminase. / Frasca, Daniela; Van der Put, Elaine; Riley, Richard L; Blomberg, Bonnie B.

In: Journal of Immunology, Vol. 172, No. 4, 15.02.2004, p. 2155-2162.

Research output: Contribution to journalArticle

@article{835a5750344343089f56a8ac15ae20c3,
title = "Reduced Ig Class Switch in Aged Mice Correlates with Decreased E47 and Activation-Induced Cytidine Deaminase",
abstract = "The capacity to class switch the IgH chain is critical to the effectiveness of humoral immune responses. We show that in vitro-stimulated splenic B cells from senescent mice are deficient in production of multiple class switch isotypes (IgG1, G2a, G3, and E), class switch recombination (CSR), and induction of the E2A-encoded transcription factor E47. E47 has previously been shown to be required for CSR, at least in part via expression of the activation-induced cytidine deaminase. Our studies show that impaired induction of E47, and subsequently activation-induced cytidine deaminase, contribute to poor CSR and production of secondary isotypes in senescence.",
author = "Daniela Frasca and {Van der Put}, Elaine and Riley, {Richard L} and Blomberg, {Bonnie B}",
year = "2004",
month = "2",
day = "15",
language = "English",
volume = "172",
pages = "2155--2162",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - Reduced Ig Class Switch in Aged Mice Correlates with Decreased E47 and Activation-Induced Cytidine Deaminase

AU - Frasca, Daniela

AU - Van der Put, Elaine

AU - Riley, Richard L

AU - Blomberg, Bonnie B

PY - 2004/2/15

Y1 - 2004/2/15

N2 - The capacity to class switch the IgH chain is critical to the effectiveness of humoral immune responses. We show that in vitro-stimulated splenic B cells from senescent mice are deficient in production of multiple class switch isotypes (IgG1, G2a, G3, and E), class switch recombination (CSR), and induction of the E2A-encoded transcription factor E47. E47 has previously been shown to be required for CSR, at least in part via expression of the activation-induced cytidine deaminase. Our studies show that impaired induction of E47, and subsequently activation-induced cytidine deaminase, contribute to poor CSR and production of secondary isotypes in senescence.

AB - The capacity to class switch the IgH chain is critical to the effectiveness of humoral immune responses. We show that in vitro-stimulated splenic B cells from senescent mice are deficient in production of multiple class switch isotypes (IgG1, G2a, G3, and E), class switch recombination (CSR), and induction of the E2A-encoded transcription factor E47. E47 has previously been shown to be required for CSR, at least in part via expression of the activation-induced cytidine deaminase. Our studies show that impaired induction of E47, and subsequently activation-induced cytidine deaminase, contribute to poor CSR and production of secondary isotypes in senescence.

UR - http://www.scopus.com/inward/record.url?scp=0842321787&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0842321787&partnerID=8YFLogxK

M3 - Article

C2 - 14764681

AN - SCOPUS:0842321787

VL - 172

SP - 2155

EP - 2162

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -