Abstract
Because dietary acid increases renal secretion of endothelin-1 (ET-1) which is synthesized by renal microvascular endothelium, we examined if reduced extracellular pH increases ET-1 secretion by cultured human renal microvascular endothelial cells (RMVECs). Confluent cells were exposed to serum-free media for 24 h, then incubated in either control, acid, or alkaline serum-free media for 12 h. Standard growth media pH was 7.2 after equilibration with 5% CO2 at 37°C and was made the pH of control media. Acid and alkaline media pH were 7.0 and 7.4, respectively. Added Hepes and Tris maintained all assigned pHs. Media ET-1 measured by RIA after column extraction was higher for RMVECs exposed to acid compared with control media (170.0±17.1 vs. 64.6±9.6 pM, P < 0.004) but those exposed to alkaline media (56.6±25.1 pM, P = NS vs. control) were not. Human aortic endothelial cells exposed to control, acid, and alkaline media had similar ET-1 (166.6±18.1, 139.3±18.5, and 205.9±25.3 pM, P = NS). The data show acid-stimulated ET-1 secretion by RMVECs but not aortic endothelial cells, demonstrating a new environmental factor that influences ET-1 secretion by renal microvascular endothelium and thereby possibly modulates endothelin-dependent processes in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 578-583 |
| Number of pages | 6 |
| Journal | Journal of Clinical Investigation |
| Volume | 101 |
| Issue number | 3 |
| State | Published - Feb 1 1998 |
| Externally published | Yes |
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Keywords
- Acid
- Aortic endothelial cells
- Glomerular endothelial cells
- Kidney
ASJC Scopus subject areas
- Medicine(all)
Cite this
Reduced extracellular pH increases endothelin-1 secretion by human renal microvascular endothelial cells. / Wesson, Donald E.; Simoni, Jan; Green, Dollie F.
In: Journal of Clinical Investigation, Vol. 101, No. 3, 01.02.1998, p. 578-583.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduced extracellular pH increases endothelin-1 secretion by human renal microvascular endothelial cells
AU - Wesson, Donald E.
AU - Simoni, Jan
AU - Green, Dollie F
PY - 1998/2/1
Y1 - 1998/2/1
N2 - Because dietary acid increases renal secretion of endothelin-1 (ET-1) which is synthesized by renal microvascular endothelium, we examined if reduced extracellular pH increases ET-1 secretion by cultured human renal microvascular endothelial cells (RMVECs). Confluent cells were exposed to serum-free media for 24 h, then incubated in either control, acid, or alkaline serum-free media for 12 h. Standard growth media pH was 7.2 after equilibration with 5% CO2 at 37°C and was made the pH of control media. Acid and alkaline media pH were 7.0 and 7.4, respectively. Added Hepes and Tris maintained all assigned pHs. Media ET-1 measured by RIA after column extraction was higher for RMVECs exposed to acid compared with control media (170.0±17.1 vs. 64.6±9.6 pM, P < 0.004) but those exposed to alkaline media (56.6±25.1 pM, P = NS vs. control) were not. Human aortic endothelial cells exposed to control, acid, and alkaline media had similar ET-1 (166.6±18.1, 139.3±18.5, and 205.9±25.3 pM, P = NS). The data show acid-stimulated ET-1 secretion by RMVECs but not aortic endothelial cells, demonstrating a new environmental factor that influences ET-1 secretion by renal microvascular endothelium and thereby possibly modulates endothelin-dependent processes in vivo.
AB - Because dietary acid increases renal secretion of endothelin-1 (ET-1) which is synthesized by renal microvascular endothelium, we examined if reduced extracellular pH increases ET-1 secretion by cultured human renal microvascular endothelial cells (RMVECs). Confluent cells were exposed to serum-free media for 24 h, then incubated in either control, acid, or alkaline serum-free media for 12 h. Standard growth media pH was 7.2 after equilibration with 5% CO2 at 37°C and was made the pH of control media. Acid and alkaline media pH were 7.0 and 7.4, respectively. Added Hepes and Tris maintained all assigned pHs. Media ET-1 measured by RIA after column extraction was higher for RMVECs exposed to acid compared with control media (170.0±17.1 vs. 64.6±9.6 pM, P < 0.004) but those exposed to alkaline media (56.6±25.1 pM, P = NS vs. control) were not. Human aortic endothelial cells exposed to control, acid, and alkaline media had similar ET-1 (166.6±18.1, 139.3±18.5, and 205.9±25.3 pM, P = NS). The data show acid-stimulated ET-1 secretion by RMVECs but not aortic endothelial cells, demonstrating a new environmental factor that influences ET-1 secretion by renal microvascular endothelium and thereby possibly modulates endothelin-dependent processes in vivo.
KW - Acid
KW - Aortic endothelial cells
KW - Glomerular endothelial cells
KW - Kidney
UR - http://www.scopus.com/inward/record.url?scp=0032005875&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032005875&partnerID=8YFLogxK
M3 - Article
VL - 101
SP - 578
EP - 583
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
SN - 0021-9738
IS - 3
ER -