Reduced extracellular pH increases endothelin-1 secretion by human renal microvascular endothelial cells

Donald E. Wesson, Jan Simoni, Dollie F Green

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Abstract

Because dietary acid increases renal secretion of endothelin-1 (ET-1) which is synthesized by renal microvascular endothelium, we examined if reduced extracellular pH increases ET-1 secretion by cultured human renal microvascular endothelial cells (RMVECs). Confluent cells were exposed to serum-free media for 24 h, then incubated in either control, acid, or alkaline serum-free media for 12 h. Standard growth media pH was 7.2 after equilibration with 5% CO2 at 37°C and was made the pH of control media. Acid and alkaline media pH were 7.0 and 7.4, respectively. Added Hepes and Tris maintained all assigned pHs. Media ET-1 measured by RIA after column extraction was higher for RMVECs exposed to acid compared with control media (170.0±17.1 vs. 64.6±9.6 pM, P < 0.004) but those exposed to alkaline media (56.6±25.1 pM, P = NS vs. control) were not. Human aortic endothelial cells exposed to control, acid, and alkaline media had similar ET-1 (166.6±18.1, 139.3±18.5, and 205.9±25.3 pM, P = NS). The data show acid-stimulated ET-1 secretion by RMVECs but not aortic endothelial cells, demonstrating a new environmental factor that influences ET-1 secretion by renal microvascular endothelium and thereby possibly modulates endothelin-dependent processes in vivo.

Original languageEnglish
Pages (from-to)578-583
Number of pages6
JournalJournal of Clinical Investigation
Volume101
Issue number3
StatePublished - Feb 1 1998
Externally publishedYes

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Keywords

  • Acid
  • Aortic endothelial cells
  • Glomerular endothelial cells
  • Kidney

ASJC Scopus subject areas

  • Medicine(all)

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