Redox changes of cultured endothelial cells and actin dynamics

Leni Moldovan, Nicanor I. Moldovan, Richard H. Sohn, Sahil A. Parikh, Pascal Goldschmidt-Clermont

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

We studied the association between the production of reactive oxygen species, actin organization, and cellular motility. We have used an endothelial cell monolayer-wounding assay to demonstrate that the cells at the margin of the wound thus created produced significantly more free radicals than did cells in distant rows. The rate of incorporation of actin monomers into filaments was fastest at the wound margin, where heightened production of free radicals was detected. We have tested the effect of decreasing reactive oxygen species production on the migration of endothelial cells and on actin polymerization. The NADPH inhibitor diphenylene iodonium and the superoxide dismutase mimetic manganese (III) tetrakis(1-methyl-4- pyridyl)porphyrin (MnTMPyP) virtually abolished cytochalasin D-inhibitable actin monomer incorporation at the fast-growing barbed ends of filaments. Moreover, endothelial cell migration within the wound was significantly retarded in the presence of both diphenylene iodonium and MnTMPyP. We conclude that migration of endothelial cells in response to loss of confluence includes the intracellular production of reactive oxygen species, which contribute to the actin cytoskeleton reorganization required for the migratory behavior of endothelial cells.

Original languageEnglish
Pages (from-to)549-557
Number of pages9
JournalCirculation Research
Volume86
Issue number5
StatePublished - Mar 17 2000
Externally publishedYes

Fingerprint

Oxidation-Reduction
Actins
Cultured Cells
Endothelial Cells
Reactive Oxygen Species
Porphyrins
Free Radicals
Wounds and Injuries
Cytochalasin D
Manganese
Actin Cytoskeleton
NADP
Polymerization
Superoxide Dismutase
Cell Movement
diphenyleneiodonium

Keywords

  • Actin
  • Endothelium
  • Migration
  • Polymerization
  • Reactive oxygen species

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Moldovan, L., Moldovan, N. I., Sohn, R. H., Parikh, S. A., & Goldschmidt-Clermont, P. (2000). Redox changes of cultured endothelial cells and actin dynamics. Circulation Research, 86(5), 549-557.

Redox changes of cultured endothelial cells and actin dynamics. / Moldovan, Leni; Moldovan, Nicanor I.; Sohn, Richard H.; Parikh, Sahil A.; Goldschmidt-Clermont, Pascal.

In: Circulation Research, Vol. 86, No. 5, 17.03.2000, p. 549-557.

Research output: Contribution to journalArticle

Moldovan, L, Moldovan, NI, Sohn, RH, Parikh, SA & Goldschmidt-Clermont, P 2000, 'Redox changes of cultured endothelial cells and actin dynamics', Circulation Research, vol. 86, no. 5, pp. 549-557.
Moldovan L, Moldovan NI, Sohn RH, Parikh SA, Goldschmidt-Clermont P. Redox changes of cultured endothelial cells and actin dynamics. Circulation Research. 2000 Mar 17;86(5):549-557.
Moldovan, Leni ; Moldovan, Nicanor I. ; Sohn, Richard H. ; Parikh, Sahil A. ; Goldschmidt-Clermont, Pascal. / Redox changes of cultured endothelial cells and actin dynamics. In: Circulation Research. 2000 ; Vol. 86, No. 5. pp. 549-557.
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