Abstract
CD4+ T cells specific for the diabetes-associated autoantigen GAD65 were analyzed using peripheral blood samples after pancreas transplantation in subjects with T1D with clinical evidence of recurrent autoimmune diabetes. MHC class II tetramers facilitated the identification and cloning of antigen-specific autoreactive cells, which were found at several time points over a multiyear span, in spite of chronic immunosuppression of the subjects. Comparisons of TCR clonotypes by cDNA sequencing revealed that identical T cells were present in the circulation, separated by long time intervals, consistent with a persistent memory response associated with recurrent autoimmunity.
Original language | English (US) |
---|---|
Pages (from-to) | 23-30 |
Number of pages | 8 |
Journal | Clinical Immunology |
Volume | 128 |
Issue number | 1 |
DOIs | |
State | Published - Jul 2008 |
Keywords
- Autoimmune disease
- CD4 T cells
- GAD65
- Islet replacement therapy
- MHC class II tetramer
- Pancreas transplantation
- Type 1 diabetes
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy