Recurrence of autoreactive antigen-specific CD4+ T cells in autoimmune diabetes after pancreas transplantation

Elsa Laughlin; George Burke; Alberto Pugliese; Ben Falk; Gerald Nepom

doi:10.1016/j.clim.2008.03.459

Recurrence of autoreactive antigen-specific CD4+ T cells in autoimmune diabetes after pancreas transplantation

Elsa Laughlin, George Burke, Alberto Pugliese, Ben Falk, Gerald Nepom

Research output: Contribution to journal › Article

48 Scopus citations

Abstract

CD4+ T cells specific for the diabetes-associated autoantigen GAD65 were analyzed using peripheral blood samples after pancreas transplantation in subjects with T1D with clinical evidence of recurrent autoimmune diabetes. MHC class II tetramers facilitated the identification and cloning of antigen-specific autoreactive cells, which were found at several time points over a multiyear span, in spite of chronic immunosuppression of the subjects. Comparisons of TCR clonotypes by cDNA sequencing revealed that identical T cells were present in the circulation, separated by long time intervals, consistent with a persistent memory response associated with recurrent autoimmunity.

Original language	English (US)
Pages (from-to)	23-30
Number of pages	8
Journal	Clinical Immunology
Volume	128
Issue number	1
DOIs	https://doi.org/10.1016/j.clim.2008.03.459
State	Published - Jul 1 2008

Keywords

Autoimmune disease
CD4 T cells
GAD65
Islet replacement therapy
MHC class II tetramer
Pancreas transplantation
Type 1 diabetes

ASJC Scopus subject areas

Immunology
Immunology and Allergy

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Laughlin, E., Burke, G., Pugliese, A., Falk, B., & Nepom, G. (2008). Recurrence of autoreactive antigen-specific CD4+ T cells in autoimmune diabetes after pancreas transplantation. Clinical Immunology, 128(1), 23-30. https://doi.org/10.1016/j.clim.2008.03.459

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