Abstract
CD4+ T cells specific for the diabetes-associated autoantigen GAD65 were analyzed using peripheral blood samples after pancreas transplantation in subjects with T1D with clinical evidence of recurrent autoimmune diabetes. MHC class II tetramers facilitated the identification and cloning of antigen-specific autoreactive cells, which were found at several time points over a multiyear span, in spite of chronic immunosuppression of the subjects. Comparisons of TCR clonotypes by cDNA sequencing revealed that identical T cells were present in the circulation, separated by long time intervals, consistent with a persistent memory response associated with recurrent autoimmunity.
| Original language | English |
|---|---|
| Pages (from-to) | 23-30 |
| Number of pages | 8 |
| Journal | Clinical Immunology |
| Volume | 128 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 2008 |
Fingerprint
Keywords
- Autoimmune disease
- CD4 T cells
- GAD65
- Islet replacement therapy
- MHC class II tetramer
- Pancreas transplantation
- Type 1 diabetes
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Recurrence of autoreactive antigen-specific CD4+ T cells in autoimmune diabetes after pancreas transplantation. / Laughlin, Elsa; Burke, George W; Pugliese, Alberto; Falk, Ben; Nepom, Gerald.
In: Clinical Immunology, Vol. 128, No. 1, 01.07.2008, p. 23-30.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Recurrence of autoreactive antigen-specific CD4+ T cells in autoimmune diabetes after pancreas transplantation
AU - Laughlin, Elsa
AU - Burke, George W
AU - Pugliese, Alberto
AU - Falk, Ben
AU - Nepom, Gerald
PY - 2008/7/1
Y1 - 2008/7/1
N2 - CD4+ T cells specific for the diabetes-associated autoantigen GAD65 were analyzed using peripheral blood samples after pancreas transplantation in subjects with T1D with clinical evidence of recurrent autoimmune diabetes. MHC class II tetramers facilitated the identification and cloning of antigen-specific autoreactive cells, which were found at several time points over a multiyear span, in spite of chronic immunosuppression of the subjects. Comparisons of TCR clonotypes by cDNA sequencing revealed that identical T cells were present in the circulation, separated by long time intervals, consistent with a persistent memory response associated with recurrent autoimmunity.
AB - CD4+ T cells specific for the diabetes-associated autoantigen GAD65 were analyzed using peripheral blood samples after pancreas transplantation in subjects with T1D with clinical evidence of recurrent autoimmune diabetes. MHC class II tetramers facilitated the identification and cloning of antigen-specific autoreactive cells, which were found at several time points over a multiyear span, in spite of chronic immunosuppression of the subjects. Comparisons of TCR clonotypes by cDNA sequencing revealed that identical T cells were present in the circulation, separated by long time intervals, consistent with a persistent memory response associated with recurrent autoimmunity.
KW - Autoimmune disease
KW - CD4 T cells
KW - GAD65
KW - Islet replacement therapy
KW - MHC class II tetramer
KW - Pancreas transplantation
KW - Type 1 diabetes
UR - http://www.scopus.com/inward/record.url?scp=49949152047&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=49949152047&partnerID=8YFLogxK
U2 - 10.1016/j.clim.2008.03.459
DO - 10.1016/j.clim.2008.03.459
M3 - Article
C2 - 18455963
AN - SCOPUS:49949152047
VL - 128
SP - 23
EP - 30
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 1
ER -