Recovery of chondrocyte metabolic activity after thermal exposure

Lee Kaplan, Constance R. Chu, James P. Bradley, Freddie H. Fu, Rebecca K. Studer

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background: The relationship between temperature elevation and thermal exposure time during thermal chondroplasty has implications for cell viability and subsequent articular cartilage function. Purpose: To characterize cartilage metabolic changes after exposure to thermal stress and to determine whether changes seen acutely are reversible. Study Design: Controlled laboratory study. Methods: Human cartilage was exposed to a 45°, 50°, or 55°C bath for up to 3 minutes. Untreated control specimens were analyzed with each group. Viability and metabolic capability of treated and untreated specimens were evaluated immediately or 1 week after thermal stress by using methylthiotetrazole conversion, 3H-serine incorporation into protein, and 35S-sulfate incorporation into newly synthesized proteoglycan. Results: Nonarthritic and arthritic articular cartilage metabolic activity declined with increasing thermal exposure. Articular cartilage displayed a recovery from thermal stress after exposure to the 50°C but not the 55°C bath. Arthritic cartilage displayed increased sensitivity with higher temperatures. Conclusions: Understanding of the increased sensitivity to thermal stress of arthritic articular cartilage may be helpful in thermally based treatments. Clinical Relevance: Further correlation with the temperatures attained during thermal chondroplasty will be necessary to confirm the clinical relevance of these in vitro observations to the use of radiofrequency energy devices to treat partial-thickness chondral lesions.

Original languageEnglish
Pages (from-to)392-398
Number of pages7
JournalAmerican Journal of Sports Medicine
Volume31
Issue number3
StatePublished - May 1 2003
Externally publishedYes

Fingerprint

Chondrocytes
Hot Temperature
Articular Cartilage
Cartilage
Arthritis
Baths
Temperature
Proteoglycans
Serine
Sulfates
Cell Survival
Equipment and Supplies

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Kaplan, L., Chu, C. R., Bradley, J. P., Fu, F. H., & Studer, R. K. (2003). Recovery of chondrocyte metabolic activity after thermal exposure. American Journal of Sports Medicine, 31(3), 392-398.

Recovery of chondrocyte metabolic activity after thermal exposure. / Kaplan, Lee; Chu, Constance R.; Bradley, James P.; Fu, Freddie H.; Studer, Rebecca K.

In: American Journal of Sports Medicine, Vol. 31, No. 3, 01.05.2003, p. 392-398.

Research output: Contribution to journalArticle

Kaplan, L, Chu, CR, Bradley, JP, Fu, FH & Studer, RK 2003, 'Recovery of chondrocyte metabolic activity after thermal exposure', American Journal of Sports Medicine, vol. 31, no. 3, pp. 392-398.
Kaplan, Lee ; Chu, Constance R. ; Bradley, James P. ; Fu, Freddie H. ; Studer, Rebecca K. / Recovery of chondrocyte metabolic activity after thermal exposure. In: American Journal of Sports Medicine. 2003 ; Vol. 31, No. 3. pp. 392-398.
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