Recombinant yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 gag induces SIV-specific CD8+ T-cell responses in rhesus macaques

Myrna C. Bonaldo, Mauricio A. Martins, Richard Rudersdorf, Philip A. Mudd, Jonah B. Sacha, Shari M. Piaskowski, Patrícia C. Costa Neves, Marlon G. Veloso De Santana, Lara Vojnov, Saverio Capuano, Eva G. Rakasz, Nancy A. Wilson, John Fulkerson, Jerald C. Sadoff, David I. Watkins, Ricardo Galler

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Here we describe a novel vaccine vector for expressing human immunodeficiency virus (HIV) antigens. We show that recombinant attenuated yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 Gag sequences can be used as a vector to generate SIV-specific CD8+ T-cell responses in the rhesus macaque. Priming with recombinant BCG expressing SIV antigens increased the frequency of these SIV-specific CD8+ T-cell responses after recombinant YF17D boosting. These recombinant YF17D-induced SIV-specific CD8+ T cells secreted several cytokines, were largely effector memory T cells, and suppressed viral replication in CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)3699-3706
Number of pages8
JournalJournal of virology
Volume84
Issue number7
DOIs
StatePublished - Apr 1 2010

    Fingerprint

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Bonaldo, M. C., Martins, M. A., Rudersdorf, R., Mudd, P. A., Sacha, J. B., Piaskowski, S. M., Costa Neves, P. C., Veloso De Santana, M. G., Vojnov, L., Capuano, S., Rakasz, E. G., Wilson, N. A., Fulkerson, J., Sadoff, J. C., Watkins, D. I., & Galler, R. (2010). Recombinant yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 gag induces SIV-specific CD8+ T-cell responses in rhesus macaques. Journal of virology, 84(7), 3699-3706. https://doi.org/10.1128/JVI.02255-09