Recombinant yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 gag induces SIV-specific CD8+ T-cell responses in rhesus macaques

Myrna C. Bonaldo, Mauricio Martins, Richard Rudersdorf, Philip A. Mudd, Jonah B. Sacha, Shari M. Piaskowski, Patrícia C. Costa Neves, Marlon G. Veloso De Santana, Lara Vojnov, Saverio Capuano, Eva G. Rakasz, Nancy A. Wilson, John Fulkerson, Jerald C. Sadoff, David Watkins, Ricardo Galler

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Here we describe a novel vaccine vector for expressing human immunodeficiency virus (HIV) antigens. We show that recombinant attenuated yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 Gag sequences can be used as a vector to generate SIV-specific CD8+ T-cell responses in the rhesus macaque. Priming with recombinant BCG expressing SIV antigens increased the frequency of these SIV-specific CD8+ T-cell responses after recombinant YF17D boosting. These recombinant YF17D-induced SIV-specific CD8+ T cells secreted several cytokines, were largely effector memory T cells, and suppressed viral replication in CD4+ T cells.

Original languageEnglish
Pages (from-to)3699-3706
Number of pages8
JournalJournal of Virology
Volume84
Issue number7
DOIs
StatePublished - Apr 1 2010
Externally publishedYes

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Yellow Fever Vaccine
Yellow fever virus
Simian immunodeficiency virus
Simian Immunodeficiency Virus
Synthetic Vaccines
Macaca mulatta
T-lymphocytes
vaccines
T-Lymphocytes
viruses
antigens
vector vaccines
Antigens
Attenuated Vaccines
Human immunodeficiency virus
Mycobacterium bovis
virus replication
cytokines
Vaccines
HIV

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Recombinant yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 gag induces SIV-specific CD8+ T-cell responses in rhesus macaques. / Bonaldo, Myrna C.; Martins, Mauricio; Rudersdorf, Richard; Mudd, Philip A.; Sacha, Jonah B.; Piaskowski, Shari M.; Costa Neves, Patrícia C.; Veloso De Santana, Marlon G.; Vojnov, Lara; Capuano, Saverio; Rakasz, Eva G.; Wilson, Nancy A.; Fulkerson, John; Sadoff, Jerald C.; Watkins, David; Galler, Ricardo.

In: Journal of Virology, Vol. 84, No. 7, 01.04.2010, p. 3699-3706.

Research output: Contribution to journalArticle

Bonaldo, MC, Martins, M, Rudersdorf, R, Mudd, PA, Sacha, JB, Piaskowski, SM, Costa Neves, PC, Veloso De Santana, MG, Vojnov, L, Capuano, S, Rakasz, EG, Wilson, NA, Fulkerson, J, Sadoff, JC, Watkins, D & Galler, R 2010, 'Recombinant yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 gag induces SIV-specific CD8+ T-cell responses in rhesus macaques', Journal of Virology, vol. 84, no. 7, pp. 3699-3706. https://doi.org/10.1128/JVI.02255-09
Bonaldo, Myrna C. ; Martins, Mauricio ; Rudersdorf, Richard ; Mudd, Philip A. ; Sacha, Jonah B. ; Piaskowski, Shari M. ; Costa Neves, Patrícia C. ; Veloso De Santana, Marlon G. ; Vojnov, Lara ; Capuano, Saverio ; Rakasz, Eva G. ; Wilson, Nancy A. ; Fulkerson, John ; Sadoff, Jerald C. ; Watkins, David ; Galler, Ricardo. / Recombinant yellow fever vaccine virus 17D expressing simian immunodeficiency virus SIVmac239 gag induces SIV-specific CD8+ T-cell responses in rhesus macaques. In: Journal of Virology. 2010 ; Vol. 84, No. 7. pp. 3699-3706.
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