Recombinant rabbit secretory immunoglobulin molecules: alpha chains with maternal (paternal) variable region allotypes and paternal (maternal) constant region allotypes

K. L. Knight, T. R. Malek, W. C. Hanly

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27 Scopus citations

Abstract

A population of IgA molecules having heavy chains coded by two parental chromosomes in trans position was identified in rabbits heterozygous at both the V(H)a locus, which controls allotypic specificities on the variable part of heavy chains, and the C(α)g locus, which controls allotypic specificities on the constant part of alpha chains. These recombinant molecules have alpha chain allotypic specificities controlled by both the maternal V(H)a gene and the paternal C(α)g gene or conversely, the paternal V(H)a gene and the maternal C(α)g gene. These recombinant molecules were found in F(ab)(2α) fractions obtained after passage of F(ab)(2α) preparations through immunosorbent columns designed to remove one population of F(ab)(2α) molecules, i.e., g74 or g75 type molecules. The effluent F(ab)(2α) fractions were then examined by radio precipitation methods for allotypic specificities controlled by the V(H)a and C(α)g loci. About 40% of the g75 F(ab)2α) molecules from each of three rabbits with the a1g74 and a2g75 allogroups were alg75 recombinants. These alg75 recombinant molecules represented from 2.5-5.6% of the total unfractionated F(ab)(2α) sample. The F(ab)(2α) fractions from two rabbits with the a1g75 and a3g74 allogroups had from 1.8-8.2% recombinant molecules: some were alg74 recombinants and some were a3g75 recombinants. Somatic recombination as a mechanism responsible for the synthesis of polypeptide chains in which part of the information is obtained from one chromosome and part from the homologous chromosome is discussed.

Original languageEnglish (US)
Pages (from-to)1169-1173
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume71
Issue number4
DOIs
StatePublished - 1974
Externally publishedYes

ASJC Scopus subject areas

  • General

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