Recombinant protein-co-PEG networks as cell-adhesive and proteolytically degradable hydrogel matrixes. Part I: Development and physicochemical characteristics

Simone C. Rizzi, Jeffrey A. Hubbell

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Toward the development of synthetic bioactive materials to support tissue repair, we present here the design, production, and characterization of genetically engineered protein polymers carrying specific key features of the natural extracellular matrix, as well as cross-linking with functionalized poly(ethylene glycol) (PEG) to form hybrid hydrogel networks. The repeating units of target recombinant protein polymers contain a cell-binding site for ligation of cell-surface integrin receptors and substrates for plasmin and matrix metalloproteinases (MMPs), proteases implicated in wound healing and tissue regeneration. Hydrogels were formed under physiological conditions via Michael-type conjugate addition of vinyl sulfone groups of end-functionalized PEG with thiols of cysteine residues, representing designed chemical cross-linking sites within recombinant proteins. Cross-linking kinetics was shown to increase with the pH of precursor solutions. The elastic moduli (G′) and swelling ratios (Qm) of the resulting hydrogels could be varied as a function of the stoichiometry of the reacting groups and precursor concentration. Optima of G′ and Qm, maximum and minimum, respectively, were obtained at stoichiometry ratios r slightly in excess of 1 (r = cysteine/vinyl sulfone). The pool of technologies utilized here represents a promising approach for the development of artificial matrixes tailored for specific medical applications.

Original languageEnglish
Pages (from-to)1226-1238
Number of pages13
JournalBiomacromolecules
Volume6
Issue number3
DOIs
StatePublished - May 1 2005
Externally publishedYes

Fingerprint

Recombinant proteins
Hydrogels
Hydrogel
Recombinant Proteins
Adhesives
Polyethylene glycols
Cysteine
Polymers
Stoichiometry
Ethylene Glycol
Elastic Modulus
Fibrinolysin
Cell Surface Receptors
Matrix Metalloproteinases
Sulfhydryl Compounds
Integrins
Wound Healing
Extracellular Matrix
Ligation
Regeneration

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Recombinant protein-co-PEG networks as cell-adhesive and proteolytically degradable hydrogel matrixes. Part I : Development and physicochemical characteristics. / Rizzi, Simone C.; Hubbell, Jeffrey A.

In: Biomacromolecules, Vol. 6, No. 3, 01.05.2005, p. 1226-1238.

Research output: Contribution to journalArticle

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