Recombinant DNA strategies in genetic neurological diseases

Allen D. Roses, Margaret A. Pericak‐Vance, Larry H. Yamaoka, Elton Stubblefield, Jeffery Stajich, Jeffery M. Vance, Marcia J. Roses, Donald B. Carter

Research output: Contribution to journalReview articlepeer-review

16 Scopus citations

Abstract

The application of recombinant DNA techniques applied to the study of genetic neurological diseases will play a major role in the practice of neurology in upcoming years. Strategies are now available to develop useful and relatively simple biochemical diagnostic tests for heterozygous individuals with diseases inherited as autosomal dominant traits. In addition, molecular genetic methods will lead to the delineation of the genomic mutations responsible for these diseases. This review will update the current status of research in several neurological genetic diseases including myotonic muscular dystrophy, Huntington's disease, Charcot-Marie-Tooth disease and Duchenne muscular dystrophy (X-linked). An introduction and overview of the methodology is provided. Specific research strategies including random screeening of libraries, chromosome walking, messenger RNA selection, and messenger RNA translation are described. These strategies are designed to provide heterozygote identification, prenatal diagnosis and gestational management, the development of rational therapies, and the understanding of the molecular basis of disease expression.

Original languageEnglish (US)
Pages (from-to)339-355
Number of pages17
JournalMuscle & Nerve
Volume6
Issue number5
DOIs
StatePublished - Jun 1983
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

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