Receptor tyrosine phosphatase-δ is a homophilic, neurite-promoting cell adhesion molecule for CNS neurons

J. Wang, J. L. Bixby

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

Appropriate regulation of tyrosine phosphorylation is essential for axon growth and guidance; evidence from invertebrates indicates that receptor-type tyrosine phosphatases (RPTPs) are required for correct axon growth during CNS development. One vertebrate RPTP, PTP-δ, is highly expressed in brain and has a cell adhesion molecule-like extracellular domain (ECD) comprising three immunoglobulin repeats and eight fibronectin type III repeats. Using fluorescent beads (Covaspheres) coated with the PTP-δ ECD, as well as insect cells expressing PTP-δ on their surfaces, we show that PTP-δ is a homophilic cell adhesion molecule. A variety of chick neurons adhere strongly to an Fc fusion protein containing the PTP-δ ECD. Additionally, substrate- bound PTP-δ ECD fusion protein strongly promotes neurite outgrowth from forebrain neurons; this effect is separable from its effect on adhesion. Our results indicate that PTP-δ is a neurite-promoting cell adhesion molecule for CNS neurons.

Original languageEnglish (US)
Pages (from-to)370-384
Number of pages15
JournalMolecular and Cellular Neurosciences
Volume14
Issue number4-5
DOIs
StatePublished - Oct 1999

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ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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