Receptor tyrosine phosphatase-δ is a homophilic, neurite-promoting cell adhesion molecule for CNS neurons

J. Wang, John Bixby

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Appropriate regulation of tyrosine phosphorylation is essential for axon growth and guidance; evidence from invertebrates indicates that receptor-type tyrosine phosphatases (RPTPs) are required for correct axon growth during CNS development. One vertebrate RPTP, PTP-δ, is highly expressed in brain and has a cell adhesion molecule-like extracellular domain (ECD) comprising three immunoglobulin repeats and eight fibronectin type III repeats. Using fluorescent beads (Covaspheres) coated with the PTP-δ ECD, as well as insect cells expressing PTP-δ on their surfaces, we show that PTP-δ is a homophilic cell adhesion molecule. A variety of chick neurons adhere strongly to an Fc fusion protein containing the PTP-δ ECD. Additionally, substrate- bound PTP-δ ECD fusion protein strongly promotes neurite outgrowth from forebrain neurons; this effect is separable from its effect on adhesion. Our results indicate that PTP-δ is a neurite-promoting cell adhesion molecule for CNS neurons.

Original languageEnglish
Pages (from-to)370-384
Number of pages15
JournalMolecular and Cellular Neurosciences
Volume14
Issue number4-5
DOIs
StatePublished - Oct 1 1999

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Cell Adhesion Molecules
Neurites
Phosphoric Monoester Hydrolases
Neurons
Invertebrates
Growth
Prosencephalon
Fibronectins
Axons
Tyrosine
Insects
Vertebrates
Immunoglobulins
Phosphorylation
Brain
tyrosine receptor
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Receptor tyrosine phosphatase-δ is a homophilic, neurite-promoting cell adhesion molecule for CNS neurons. / Wang, J.; Bixby, John.

In: Molecular and Cellular Neurosciences, Vol. 14, No. 4-5, 01.10.1999, p. 370-384.

Research output: Contribution to journalArticle

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