Receptor-mediated Mn2+ influx in rat hepatocytes: Comparison of cells loaded with Fura-2 ester and cells microinjected with Fura-2 salt

G. E.N. Kass, D. L. Webb, S. C. Chow, J. Llopis, P. O. Berggren

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+. Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl)hydroquinone or thapsigargin did not. The pattern of Fura-2 quenching by Mn2+ only correlated with the movement of Mn2+ across the plasma membrane.

Original languageEnglish (US)
Pages (from-to)5-9
Number of pages5
JournalBiochemical Journal
Volume302
Issue number1
DOIs
StatePublished - 1994

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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