Receptive range analysis of a mouse odorant receptor subfamily

Jingyi Li, Rafi Haddad, Vanessa Santos, Selvan Bavan, Charles W. Luetje

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Mammals deploy a large array of odorant receptors (ORs) to detect and distinguish a vast number of odorant molecules. ORs vary widely in the type of odorant structures recognized and in the breadth of molecular receptive range (MRR), with some ORs recognizing a small group of closely related molecules and other ORs recognizing a wide range of structures. While closely related ORs have been shown to have similar MRRs, the functional relationships among less closely related ORs are unclear. We screened a small group of ORs with a diverse odorant panel to identify a new odorant-OR pairing (unsaturated aldehydes and MOR263-3). We then extensively screened MOR263-3 and a series of additional MORs related to MOR263-3 in various ways. MORs related by phylogenetic analysis (several other members of the MOR263 subfamily) had MRRs that overlapped with the MRR of MOR263-3, even with amino acid identity as low as 48% (MOR263-2). MOR171-17, predicted to be functionally related to MOR263-3 by an alternative bioinformatic analysis, but with only 39% amino acid identity, had a distinct odorant specificity. Our results support the use of phylogenetic analysis to predict functional relationships among ORs with relatively low amino acid identity.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalJournal of neurochemistry
Volume134
Issue number1
DOIs
StatePublished - Jul 1 2015

Keywords

  • Xenopus oocytes
  • electrophysiology
  • heterologous expression
  • ligand specificity
  • molecular receptive range
  • olfactory receptors

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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