Abstract
Stimulant use may accelerate HIV disease progression through biological and behavioral pathways. However, scant research with treated HIV-positive persons has examined stimulant-associated alterations in pathophysiologic processes relevant to HIV pathogenesis. In a sample of 55 HIV-positive, methamphetamine-using sexual minority men with a viral load less than 200 copies/mL, we conducted RNA sequencing to examine patterns of leukocyte gene expression in participants who had a urine sample that was reactive for stimulants (n = 27) as compared to those who tested non-reactive (n = 28). Results indicated differential expression of 32 genes and perturbation of 168 pathways in recent stimulant users. We observed statistically significant differential expression of single genes previously associated with HIV latency, cell cycle regulation, and immune activation in recent stimulant users (false discovery rate p < 0.10). Pathway analyses indicated enrichment for genes associated with inflammation, innate immune activation, neuroendocrine hormone regulation, and neurotransmitter synthesis. Recent stimulant users displayed concurrent elevations in plasma levels of tumor necrosis factor – alpha (TNF-α) but not interleukin 6 (IL-6). Further research is needed to examine the bio-behavioral mechanisms whereby stimulant use may contribute to HIV persistence and disease progression.
| Original language | English (US) |
|---|---|
| Journal | Brain, Behavior, and Immunity |
| DOIs | |
| State | Accepted/In press - Jan 1 2018 |
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Keywords
- Cocaine
- Gene expression
- HIV
- Immune activation
- Inflammation
- Methamphetamine
- Reservoir
ASJC Scopus subject areas
- Immunology
- Endocrine and Autonomic Systems
- Behavioral Neuroscience
Cite this
Recent stimulant use and leukocyte gene expression in methamphetamine users with treated HIV infection. / Carrico, Adam; Flentje, Annesa; Kober, Kord; Lee, Sulggi; Hunt, Peter; Riley, Elise D.; Shoptaw, Steven; Flowers, Elena; Dilworth, Samantha E.; Pahwa, Savita G; Aouizerat, Bradley E.
In: Brain, Behavior, and Immunity, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Recent stimulant use and leukocyte gene expression in methamphetamine users with treated HIV infection
AU - Carrico, Adam
AU - Flentje, Annesa
AU - Kober, Kord
AU - Lee, Sulggi
AU - Hunt, Peter
AU - Riley, Elise D.
AU - Shoptaw, Steven
AU - Flowers, Elena
AU - Dilworth, Samantha E.
AU - Pahwa, Savita G
AU - Aouizerat, Bradley E.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Stimulant use may accelerate HIV disease progression through biological and behavioral pathways. However, scant research with treated HIV-positive persons has examined stimulant-associated alterations in pathophysiologic processes relevant to HIV pathogenesis. In a sample of 55 HIV-positive, methamphetamine-using sexual minority men with a viral load less than 200 copies/mL, we conducted RNA sequencing to examine patterns of leukocyte gene expression in participants who had a urine sample that was reactive for stimulants (n = 27) as compared to those who tested non-reactive (n = 28). Results indicated differential expression of 32 genes and perturbation of 168 pathways in recent stimulant users. We observed statistically significant differential expression of single genes previously associated with HIV latency, cell cycle regulation, and immune activation in recent stimulant users (false discovery rate p < 0.10). Pathway analyses indicated enrichment for genes associated with inflammation, innate immune activation, neuroendocrine hormone regulation, and neurotransmitter synthesis. Recent stimulant users displayed concurrent elevations in plasma levels of tumor necrosis factor – alpha (TNF-α) but not interleukin 6 (IL-6). Further research is needed to examine the bio-behavioral mechanisms whereby stimulant use may contribute to HIV persistence and disease progression.
AB - Stimulant use may accelerate HIV disease progression through biological and behavioral pathways. However, scant research with treated HIV-positive persons has examined stimulant-associated alterations in pathophysiologic processes relevant to HIV pathogenesis. In a sample of 55 HIV-positive, methamphetamine-using sexual minority men with a viral load less than 200 copies/mL, we conducted RNA sequencing to examine patterns of leukocyte gene expression in participants who had a urine sample that was reactive for stimulants (n = 27) as compared to those who tested non-reactive (n = 28). Results indicated differential expression of 32 genes and perturbation of 168 pathways in recent stimulant users. We observed statistically significant differential expression of single genes previously associated with HIV latency, cell cycle regulation, and immune activation in recent stimulant users (false discovery rate p < 0.10). Pathway analyses indicated enrichment for genes associated with inflammation, innate immune activation, neuroendocrine hormone regulation, and neurotransmitter synthesis. Recent stimulant users displayed concurrent elevations in plasma levels of tumor necrosis factor – alpha (TNF-α) but not interleukin 6 (IL-6). Further research is needed to examine the bio-behavioral mechanisms whereby stimulant use may contribute to HIV persistence and disease progression.
KW - Cocaine
KW - Gene expression
KW - HIV
KW - Immune activation
KW - Inflammation
KW - Methamphetamine
KW - Reservoir
UR - http://www.scopus.com/inward/record.url?scp=85046117881&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046117881&partnerID=8YFLogxK
U2 - 10.1016/j.bbi.2018.04.004
DO - 10.1016/j.bbi.2018.04.004
M3 - Article
JO - Brain, Behavior, and Immunity
JF - Brain, Behavior, and Immunity
SN - 0889-1591
ER -