Rearrangement and expression of T-cell receptor genes in marmoset lymphoid cell lines with natural killer activity

Mario Stevenson, D. R. Johnson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To study the role of the T-3/T-cell receptor complex in mediating natural killer activity, we have examined T-cell receptor gene configuration and expression in five transformed marmoset cell lines with natural killer activity. All lines showed rearrangement of the beta chain of the T-cell receptor and expressed full size beta chain transcripts as well as full size delta chain transcripts. Only three lines contained full size T-cell receptor alpha chain transcripts. Of the three lines which had potential for encoding functional T-3/T-cell receptor complex, those which possessed natural killer activity also expressed T-gamma transcripts. In addition, one clone with high natural killer activity which did express high levels of T-gamma mRNA was unable to produce functional T-3/T-cell receptor complex due to truncated T-cell receptor alpha chain message. The results in this paper provide strong evidence that natural killer activity is dependent upon expression of the T-cell receptor gamma chain.

Original languageEnglish
Pages (from-to)9-17
Number of pages9
JournalViral Immunology
Volume3
Issue number1
StatePublished - Oct 19 1990
Externally publishedYes

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Antigen Receptors, T-Cell, alpha-beta
T-Cell Receptor Genes
Callithrix
T-Cell Antigen Receptor
Lymphocytes
Cell Line
Antigen Receptors, T-Cell, gamma-delta
Transformed Cell Line
Clone Cells
Gene Expression
Messenger RNA

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Rearrangement and expression of T-cell receptor genes in marmoset lymphoid cell lines with natural killer activity. / Stevenson, Mario; Johnson, D. R.

In: Viral Immunology, Vol. 3, No. 1, 19.10.1990, p. 9-17.

Research output: Contribution to journalArticle

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