Rearrangement and expression of T-cell receptor genes in marmoset lymphoid cell lines with natural killer activity

M. Stevenson, D. R. Johnson

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

To study the role of the T-3/T-cell receptor complex in mediating natural killer activity, we have examined T-cell receptor gene configuration and expression in five transformed marmoset cell lines with natural killer activity. All lines showed rearrangement of the beta chain of the T-cell receptor and expressed full size beta chain transcripts as well as full size delta chain transcripts. Only three lines contained full size T-cell receptor alpha chain transcripts. Of the three lines which had potential for encoding functional T-3/T-cell receptor complex, those which possessed natural killer activity also expressed T-gamma transcripts. In addition, one clone with high natural killer activity which did express high levels of T-gamma mRNA was unable to produce functional T-3/T-cell receptor complex due to truncated T-cell receptor alpha chain message. The results in this paper provide strong evidence that natural killer activity is dependent upon expression of the T-cell receptor gamma chain.

Original languageEnglish (US)
Pages (from-to)9-17
Number of pages9
JournalViral Immunology
Volume3
Issue number1
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Molecular Medicine
  • Virology

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