Reactive oxygen species mediate activity-dependent neuron-glia signaling in output fibers of the hippocampus

Coleen M. Atkins, J. David Sweatt

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

Nonsynaptic signaling is becoming increasingly appreciated in studies of activity-dependent changes in the nervous system. We investigated the types of neuronal activity that elicit nonsynaptic communication between neurons and glial cells in hippocampal output fibers. High-frequency, but not low- frequency, action potential firing in myelinated CA1 axons of the hippocampus resulted in increased phosphorylation of the oligodendrocyte-specific protein myelin basic protein (MBP). This change was blocked by tetrodotoxin, indicating that axonally generated action potentials were necessary to regulate the phosphorylation state of MBP. Furthermore, scavengers of the reactive oxygen species superoxide and hydrogen peroxide and nitric oxide synthase inhibitors prevented activation of this neuron-glia signaling pathway. These results indicate that, during periods of increased neuronal activity in area CA1 of the hippocampus, reactive oxygen and nitrogen species are generated, which diffuse to neighboring oligodendrocytes and result in post-translational modifications of MBP, a key structural protein in myelin. Thus, in addition to their well-known capacity for activity-dependent neuron- neuron signaling, hippocampal pyramidal neurons possess a mechanism for activity-dependent neuron-glia signaling.

Original languageEnglish (US)
Pages (from-to)7241-7248
Number of pages8
JournalJournal of Neuroscience
Volume19
Issue number17
DOIs
StatePublished - Sep 1 1999

Keywords

  • Alveus
  • Glia
  • Hippocampus
  • Hydrogen peroxide
  • Myelin
  • Myelin basic protein
  • Neuron-glia signaling
  • Nitric oxide
  • Oligodendrocyte
  • Reactive oxygen species
  • Superoxide

ASJC Scopus subject areas

  • Neuroscience(all)

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