Reactive oxygen species and hyaluronidase 2 regulate airway epithelial hyaluronan fragmentation

Maria E. Monzon, Nevis L. Fregien, Nathalie Schmid, Nieves S. Falcon, Michael A Campos, S. Marina Casalino-Matsuda, Rosanna Malbran Forteza

Research output: Contribution to journalArticle

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Abstract

Hyaluronidase 2 (Hyal2) is a hyaluronan (HA)-degrading enzyme found intracellularly or/and anchored to the plasma membrane through glycosylphosphatidylinositol (GPI). Normal human bronchial epithelial cells (NHBE) grown at the air-liquid interphase (ALI), treated with PI-specific phospholipase C (PI-PLC), exhibited increased Hyal activity in secretions and decreased protein and activity on the apical membrane, confirming that GPI-anchored Hyal2 is expressed in NHBE cells and it remains active in its soluble form. We have reported that HA degradation was mediated by reactive oxygen species (ROS) in human airways. Here we show that ROS increase Hyal2 expression and activity in NHBE cells and that the p38MAPK signaling pathway is involved in this effect. Hyal2 induction was confirmed by using small interfering RNA (siRNA) expressing lentivirus. These in vitro findings correlated in vivo with smokers, where increased Hyal2 immunoreactivity in the epithelium was associated with augmented levels of HA and the appearance of low molecular mass HA species in bronchial secretions. In summary, this work provides evidence that ROS induce Hyal2, suggesting that Hyal2 is likely responsible for the sustained HA fragmentation in the airway lumen observed in inflammatory conditions associated with oxidative stress.

Original languageEnglish
Pages (from-to)26126-26134
Number of pages9
JournalJournal of Biological Chemistry
Volume285
Issue number34
DOIs
StatePublished - Aug 20 2010

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Hyaluronoglucosaminidase
Hyaluronic Acid
Reactive Oxygen Species
Glycosylphosphatidylinositols
Epithelial Cells
Lentivirus
Oxidative stress
Interphase
Molecular mass
Type C Phospholipases
Cell membranes
Small Interfering RNA
Oxidative Stress
Epithelium
Air
Cell Membrane
Membranes
Degradation
Liquids
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Reactive oxygen species and hyaluronidase 2 regulate airway epithelial hyaluronan fragmentation. / Monzon, Maria E.; Fregien, Nevis L.; Schmid, Nathalie; Falcon, Nieves S.; Campos, Michael A; Casalino-Matsuda, S. Marina; Forteza, Rosanna Malbran.

In: Journal of Biological Chemistry, Vol. 285, No. 34, 20.08.2010, p. 26126-26134.

Research output: Contribution to journalArticle

Monzon, Maria E. ; Fregien, Nevis L. ; Schmid, Nathalie ; Falcon, Nieves S. ; Campos, Michael A ; Casalino-Matsuda, S. Marina ; Forteza, Rosanna Malbran. / Reactive oxygen species and hyaluronidase 2 regulate airway epithelial hyaluronan fragmentation. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 34. pp. 26126-26134.
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