Reactions at the 3′ terminus of transfer ribonucleic acid. IX. tRNA nucleotidyltransferase activity in bacteriophage T4-infected Escherichia coli

Richard H. Hilderman, Murray P Deutscher

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

There was no detectable increase in tRNA nucleotidyltransferase activity upon infection of Escherichia coli A19 with bacteriophage T4. Three mutant strains which contained low levels of tRNA nucleotidyltransferase activity also showed no increase in activity after infection. tRNA nucleotidyltransferase was purified from both uninfected and T4-infected cells and examined for possible modification. It was found that enzyme purified from both types of cells eluted from DEAE cellulose at the same specific conductivity. In addition, the molecular weight of tRNA nucleotidyltransferase purified from both uninfected and T4-infected cells was approximately 45,000 daltons as determined by chromatography on Sephadex G-100. These results suggest that T4-infection does not lead to synthesis of a new virus-specific tRNA nucleotidyltransferase nor does it cause modification of the host enzyme.

Original languageEnglish
Pages (from-to)205-215
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume54
Issue number1
DOIs
StatePublished - Sep 5 1973
Externally publishedYes

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Bacteriophage T4
Bacteriophages
Transfer RNA
Escherichia coli
CD4-Positive T-Lymphocytes
Escherichia coli Infections
DEAE-Cellulose
Enzymes
Chromatography
Infection
Viruses
Molecular Weight
Molecular weight
tRNA nucleotidyltransferase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Reactions at the 3′ terminus of transfer ribonucleic acid. IX. tRNA nucleotidyltransferase activity in bacteriophage T4-infected Escherichia coli. / Hilderman, Richard H.; Deutscher, Murray P.

In: Biochemical and Biophysical Research Communications, Vol. 54, No. 1, 05.09.1973, p. 205-215.

Research output: Contribution to journalArticle

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