Rat model of photochemically-induced posterior ischemic optic neuropathy

Yan Wang, Dale P. Brown, Brant D. Watson, Jeffrey L. Goldberg

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Posterior Ischemic optic neuropathy (PION) is a sight-devastating disease in clinical practice. However, its pathogenesis and natural history have remained poorly understood. Recently, we developed a reliable, reproducible animal model of PION and tested the treatment effect of some neurotrophic factors in this model1. The purpose of this video is to demonstrate our photochemically induced model of posterior ischemic optic neuropathy, and to evaluate its effects with retrograde labeling of retinal ganglion cells. Following surgical exposure of the posterior optic nerve, a photosensitizing dye, erythrosin B, is intravenously injected and a laser beam is focused onto the optic nerve surface. Photochemical interaction of erythrosin B and the laser during irradiation damages the vascular endothelium, prompting microvascular occlusion mediated by platelet thrombosis and edematous compression. The resulting ischemic injury yields a gradual but pronounced retinal ganglion cell dieback, owing to a loss of axonal input - a remote, injury-induced and clinically relevant outcome. Thus, this model provides a novel platform to study the pathophysiologic course of PION, and can be further optimized for testing therapeutic approaches for optic neuropathies as well as other CNS ischemic diseases.

Original languageEnglish (US)
Article numbere52402
JournalJournal of Visualized Experiments
Volume2015
Issue number105
DOIs
StatePublished - Nov 29 2015
Externally publishedYes

Fingerprint

Ischemic Optic Neuropathy
Rats
Optics
Erythrosine
Retinal Ganglion Cells
Optic Nerve
Lasers
Optic Nerve Diseases
Central Nervous System Diseases
Vascular Endothelium
Nerve Growth Factors
Wounds and Injuries
Natural History
Thrombosis
Coloring Agents
Blood Platelets
Animal Models
Laser beam effects
Platelets
Labeling

Keywords

  • Animal model
  • Ischemic
  • Issue 105
  • Medicine
  • Optic nerve
  • Photo-chemical
  • Retinal ganglion cell
  • Survival

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Wang, Y., Brown, D. P., Watson, B. D., & Goldberg, J. L. (2015). Rat model of photochemically-induced posterior ischemic optic neuropathy. Journal of Visualized Experiments, 2015(105), [e52402]. https://doi.org/10.3791/52402

Rat model of photochemically-induced posterior ischemic optic neuropathy. / Wang, Yan; Brown, Dale P.; Watson, Brant D.; Goldberg, Jeffrey L.

In: Journal of Visualized Experiments, Vol. 2015, No. 105, e52402, 29.11.2015.

Research output: Contribution to journalArticle

Wang, Yan ; Brown, Dale P. ; Watson, Brant D. ; Goldberg, Jeffrey L. / Rat model of photochemically-induced posterior ischemic optic neuropathy. In: Journal of Visualized Experiments. 2015 ; Vol. 2015, No. 105.
@article{4885e54f297648f493a2c01e462a2732,
title = "Rat model of photochemically-induced posterior ischemic optic neuropathy",
abstract = "Posterior Ischemic optic neuropathy (PION) is a sight-devastating disease in clinical practice. However, its pathogenesis and natural history have remained poorly understood. Recently, we developed a reliable, reproducible animal model of PION and tested the treatment effect of some neurotrophic factors in this model1. The purpose of this video is to demonstrate our photochemically induced model of posterior ischemic optic neuropathy, and to evaluate its effects with retrograde labeling of retinal ganglion cells. Following surgical exposure of the posterior optic nerve, a photosensitizing dye, erythrosin B, is intravenously injected and a laser beam is focused onto the optic nerve surface. Photochemical interaction of erythrosin B and the laser during irradiation damages the vascular endothelium, prompting microvascular occlusion mediated by platelet thrombosis and edematous compression. The resulting ischemic injury yields a gradual but pronounced retinal ganglion cell dieback, owing to a loss of axonal input - a remote, injury-induced and clinically relevant outcome. Thus, this model provides a novel platform to study the pathophysiologic course of PION, and can be further optimized for testing therapeutic approaches for optic neuropathies as well as other CNS ischemic diseases.",
keywords = "Animal model, Ischemic, Issue 105, Medicine, Optic nerve, Photo-chemical, Retinal ganglion cell, Survival",
author = "Yan Wang and Brown, {Dale P.} and Watson, {Brant D.} and Goldberg, {Jeffrey L.}",
year = "2015",
month = "11",
day = "29",
doi = "10.3791/52402",
language = "English (US)",
volume = "2015",
journal = "Journal of visualized experiments : JoVE",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "105",

}

TY - JOUR

T1 - Rat model of photochemically-induced posterior ischemic optic neuropathy

AU - Wang, Yan

AU - Brown, Dale P.

AU - Watson, Brant D.

AU - Goldberg, Jeffrey L.

PY - 2015/11/29

Y1 - 2015/11/29

N2 - Posterior Ischemic optic neuropathy (PION) is a sight-devastating disease in clinical practice. However, its pathogenesis and natural history have remained poorly understood. Recently, we developed a reliable, reproducible animal model of PION and tested the treatment effect of some neurotrophic factors in this model1. The purpose of this video is to demonstrate our photochemically induced model of posterior ischemic optic neuropathy, and to evaluate its effects with retrograde labeling of retinal ganglion cells. Following surgical exposure of the posterior optic nerve, a photosensitizing dye, erythrosin B, is intravenously injected and a laser beam is focused onto the optic nerve surface. Photochemical interaction of erythrosin B and the laser during irradiation damages the vascular endothelium, prompting microvascular occlusion mediated by platelet thrombosis and edematous compression. The resulting ischemic injury yields a gradual but pronounced retinal ganglion cell dieback, owing to a loss of axonal input - a remote, injury-induced and clinically relevant outcome. Thus, this model provides a novel platform to study the pathophysiologic course of PION, and can be further optimized for testing therapeutic approaches for optic neuropathies as well as other CNS ischemic diseases.

AB - Posterior Ischemic optic neuropathy (PION) is a sight-devastating disease in clinical practice. However, its pathogenesis and natural history have remained poorly understood. Recently, we developed a reliable, reproducible animal model of PION and tested the treatment effect of some neurotrophic factors in this model1. The purpose of this video is to demonstrate our photochemically induced model of posterior ischemic optic neuropathy, and to evaluate its effects with retrograde labeling of retinal ganglion cells. Following surgical exposure of the posterior optic nerve, a photosensitizing dye, erythrosin B, is intravenously injected and a laser beam is focused onto the optic nerve surface. Photochemical interaction of erythrosin B and the laser during irradiation damages the vascular endothelium, prompting microvascular occlusion mediated by platelet thrombosis and edematous compression. The resulting ischemic injury yields a gradual but pronounced retinal ganglion cell dieback, owing to a loss of axonal input - a remote, injury-induced and clinically relevant outcome. Thus, this model provides a novel platform to study the pathophysiologic course of PION, and can be further optimized for testing therapeutic approaches for optic neuropathies as well as other CNS ischemic diseases.

KW - Animal model

KW - Ischemic

KW - Issue 105

KW - Medicine

KW - Optic nerve

KW - Photo-chemical

KW - Retinal ganglion cell

KW - Survival

UR - http://www.scopus.com/inward/record.url?scp=84949445360&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949445360&partnerID=8YFLogxK

U2 - 10.3791/52402

DO - 10.3791/52402

M3 - Article

AN - SCOPUS:84949445360

VL - 2015

JO - Journal of visualized experiments : JoVE

JF - Journal of visualized experiments : JoVE

SN - 1940-087X

IS - 105

M1 - e52402

ER -