Rat lung lectin gene expression is regulated developmentally and by dexamethasone

L. B. Clerch, P. Whitney, D. Massaro

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The cell-agglutinating activity of soluble β-galactoside-binding proteins (lectins) is developmentally regulated in several mammalian organs. Little is known of the alterations in gene expression that underlie this developmental regulation. Rat lung contains a dimeric β-galactoside-binding protein that exhibits a postnatal peak of hemagglutination activity caused in part by an increased rate of lectin synthesis. We now report rat lung lectin mRNA concentration increased to a peak at age 6 days; dexamethasone treatment aborted this increase. Southern blot analysis is compatible with the presence of more than one lectin gene. However, two lines of evidence indicate that we measured a single gene product: 1) only one lectin of subunit M(r) 14,000 is present in rat lung (Biochemistry 27: 692-699, 1988), and 2) in Northern blot analysis of RNA, the lectin cDNA hybridized with only one mRNA species. Our present findings, taken with prior studies of lectin synthesis, indicate that the postnatal increase in lectin synthesis is mediated pretranslationally and by an increased efficiency of translation. Dexamethasone treatment impairs the increase of lectin mRNA concentration but increases translational efficiency.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume256
Issue number3
StatePublished - Jan 1 1989
Externally publishedYes

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Lectins
Gene expression
Dexamethasone
Rats
Gene Expression
Lung
Galactosides
Messenger RNA
Developmental Gene Expression Regulation
Carrier Proteins
Genes
Biochemistry
Hemagglutination
Southern Blotting
Northern Blotting
Complementary DNA
RNA

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Rat lung lectin gene expression is regulated developmentally and by dexamethasone. / Clerch, L. B.; Whitney, P.; Massaro, D.

In: American Journal of Physiology - Cell Physiology, Vol. 256, No. 3, 01.01.1989.

Research output: Contribution to journalArticle

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