Rat hippocampal slices need bicarbonate for the recovery of synaptic transmission after anoxia

Eugene L. Roberts; Jiao He; Ching Ping Chih

doi:10.1016/S0006-8993(00)02587-7

Rat hippocampal slices need bicarbonate for the recovery of synaptic transmission after anoxia

Eugene L. Roberts, Jiao He, Ching Ping Chih

Neurology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The purpose of this study was to see how the nominal removal of bicarbonate (HCO₃/^-) from the extracellular space of brain tissue influenced recovery of brain tissue from anoxia. Removal of HCO₃/^- in HEPES-buffered artificial cerebrospinal fluid (aCSF) inhibited almost completely recovery of synaptic transmission in hippocampal slices after anoxia. Altered pH did not contribute to this finding because adjusting intracellular (pH(i)) and extracellular (pH(o)) pH to control levels did not reduce the effect of HCO₃/^- removal. Our results suggest that HCO₃/^- levels are important in determining the extent of anoxic or ischemic brain injury. (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	171-174
Number of pages	4
Journal	Brain research
Volume	875
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(00)02587-7
State	Published - Sep 1 2000

Keywords

Acidosis
CA1
Carboxy-SNARF-1
Extracellular pH
HEPES
Intracellular pH

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0006-8993(00)02587-7

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title = "Rat hippocampal slices need bicarbonate for the recovery of synaptic transmission after anoxia",

abstract = "The purpose of this study was to see how the nominal removal of bicarbonate (HCO3/-) from the extracellular space of brain tissue influenced recovery of brain tissue from anoxia. Removal of HCO3/- in HEPES-buffered artificial cerebrospinal fluid (aCSF) inhibited almost completely recovery of synaptic transmission in hippocampal slices after anoxia. Altered pH did not contribute to this finding because adjusting intracellular (pH(i)) and extracellular (pH(o)) pH to control levels did not reduce the effect of HCO3/- removal. Our results suggest that HCO3/- levels are important in determining the extent of anoxic or ischemic brain injury. (C) 2000 Elsevier Science B.V.",

keywords = "Acidosis, CA1, Carboxy-SNARF-1, Extracellular pH, HEPES, Intracellular pH",

author = "Roberts, {Eugene L.} and Jiao He and Chih, {Ching Ping}",

note = "Funding Information: We thank Mr. Tanner S. Sly (Department of Neurology) for his technical assistance. This work was supported by a grant from the National Institute on Aging (AG08710), and by the Office of Research and Development (R & D), Medical Research Service, Department of Veterans Affairs (VA).",

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doi = "10.1016/S0006-8993(00)02587-7",

language = "English (US)",

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T1 - Rat hippocampal slices need bicarbonate for the recovery of synaptic transmission after anoxia

AU - Roberts, Eugene L.

AU - He, Jiao

AU - Chih, Ching Ping

N1 - Funding Information: We thank Mr. Tanner S. Sly (Department of Neurology) for his technical assistance. This work was supported by a grant from the National Institute on Aging (AG08710), and by the Office of Research and Development (R & D), Medical Research Service, Department of Veterans Affairs (VA).

PY - 2000/9/1

Y1 - 2000/9/1

N2 - The purpose of this study was to see how the nominal removal of bicarbonate (HCO3/-) from the extracellular space of brain tissue influenced recovery of brain tissue from anoxia. Removal of HCO3/- in HEPES-buffered artificial cerebrospinal fluid (aCSF) inhibited almost completely recovery of synaptic transmission in hippocampal slices after anoxia. Altered pH did not contribute to this finding because adjusting intracellular (pH(i)) and extracellular (pH(o)) pH to control levels did not reduce the effect of HCO3/- removal. Our results suggest that HCO3/- levels are important in determining the extent of anoxic or ischemic brain injury. (C) 2000 Elsevier Science B.V.

AB - The purpose of this study was to see how the nominal removal of bicarbonate (HCO3/-) from the extracellular space of brain tissue influenced recovery of brain tissue from anoxia. Removal of HCO3/- in HEPES-buffered artificial cerebrospinal fluid (aCSF) inhibited almost completely recovery of synaptic transmission in hippocampal slices after anoxia. Altered pH did not contribute to this finding because adjusting intracellular (pH(i)) and extracellular (pH(o)) pH to control levels did not reduce the effect of HCO3/- removal. Our results suggest that HCO3/- levels are important in determining the extent of anoxic or ischemic brain injury. (C) 2000 Elsevier Science B.V.

KW - Acidosis

KW - CA1

KW - Carboxy-SNARF-1

KW - Extracellular pH

KW - HEPES

KW - Intracellular pH

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U2 - 10.1016/S0006-8993(00)02587-7

DO - 10.1016/S0006-8993(00)02587-7

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AN - SCOPUS:0343390315

VL - 875

SP - 171

EP - 174

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -

Rat hippocampal slices need bicarbonate for the recovery of synaptic transmission after anoxia

Abstract

Keywords

ASJC Scopus subject areas

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