Rat hippocampal slices need bicarbonate for the recovery of synaptic transmission after anoxia

Eugene L. Roberts, Jiao He, Ching Ping Chih

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The purpose of this study was to see how the nominal removal of bicarbonate (HCO3/-) from the extracellular space of brain tissue influenced recovery of brain tissue from anoxia. Removal of HCO3/- in HEPES-buffered artificial cerebrospinal fluid (aCSF) inhibited almost completely recovery of synaptic transmission in hippocampal slices after anoxia. Altered pH did not contribute to this finding because adjusting intracellular (pH(i)) and extracellular (pH(o)) pH to control levels did not reduce the effect of HCO3/- removal. Our results suggest that HCO3/- levels are important in determining the extent of anoxic or ischemic brain injury. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)171-174
Number of pages4
JournalBrain Research
Volume875
Issue number1-2
DOIs
StatePublished - Sep 1 2000

Keywords

  • Acidosis
  • CA1
  • Carboxy-SNARF-1
  • Extracellular pH
  • HEPES
  • Intracellular pH

ASJC Scopus subject areas

  • Neuroscience(all)

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