Abstract
A novel method is presented for immobilizing rare single cells on MEMS scale sensor sites. The presented method does not require immobilization site to be chemically functionalized. Immobilizing single cells precisely on MEMS sensor sites is a requirement for many single cell analysis methods - reliability of cell analysis directly depends on the reliability of immobilization. The presented method uses magnetic forces to immobilize cells labeled with superparamagnetic beads on MEMS scale sites of interest. It can be coupled with many cell analysis techniques to form MEMS lab-on-a-chip devices due to the simple fabrication setup and principle of operation. The performance of the device was demonstrated with unbound superparamagnetic beads as well as with human breast tumor cells from the cell line MCF-7. Immobilization percentage and area were 95.2% and 1000 μm2, respectively, for superparamagnetic beads. MCF-7 cells labeled with superparamagnetic beads were immobilized at a percentage of 69.2% with the average distance from immobilized cells to micro-electromagnet being 50.8 μm. Thus, the presented method is a good candidate for integration with microfluidic sensing and analysis platforms.
| Original language | English (US) |
|---|---|
| Article number | 7544558 |
| Pages (from-to) | 7572-7580 |
| Number of pages | 9 |
| Journal | IEEE Sensors Journal |
| Volume | 16 |
| Issue number | 21 |
| DOIs | |
| State | Published - Nov 1 2016 |
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Keywords
- Cell immobilization
- circulating tumor cells
- microfluidics
- single cell analysis
- superparamagnetic beads
ASJC Scopus subject areas
- Instrumentation
- Electrical and Electronic Engineering
Cite this
Rare Cell Immobilization on MEMS Scale Sensors Using Micro-Electromagnets. / Gajasinghe, Rajapaksha W R L; Toprak, Alperen; Senveli, Sukru U.; Yildirim, Yagmur Akin; Jones, Michelle; Ince, Tan; Tigli, Onur.
In: IEEE Sensors Journal, Vol. 16, No. 21, 7544558, 01.11.2016, p. 7572-7580.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rare Cell Immobilization on MEMS Scale Sensors Using Micro-Electromagnets
AU - Gajasinghe, Rajapaksha W R L
AU - Toprak, Alperen
AU - Senveli, Sukru U.
AU - Yildirim, Yagmur Akin
AU - Jones, Michelle
AU - Ince, Tan
AU - Tigli, Onur
PY - 2016/11/1
Y1 - 2016/11/1
N2 - A novel method is presented for immobilizing rare single cells on MEMS scale sensor sites. The presented method does not require immobilization site to be chemically functionalized. Immobilizing single cells precisely on MEMS sensor sites is a requirement for many single cell analysis methods - reliability of cell analysis directly depends on the reliability of immobilization. The presented method uses magnetic forces to immobilize cells labeled with superparamagnetic beads on MEMS scale sites of interest. It can be coupled with many cell analysis techniques to form MEMS lab-on-a-chip devices due to the simple fabrication setup and principle of operation. The performance of the device was demonstrated with unbound superparamagnetic beads as well as with human breast tumor cells from the cell line MCF-7. Immobilization percentage and area were 95.2% and 1000 μm2, respectively, for superparamagnetic beads. MCF-7 cells labeled with superparamagnetic beads were immobilized at a percentage of 69.2% with the average distance from immobilized cells to micro-electromagnet being 50.8 μm. Thus, the presented method is a good candidate for integration with microfluidic sensing and analysis platforms.
AB - A novel method is presented for immobilizing rare single cells on MEMS scale sensor sites. The presented method does not require immobilization site to be chemically functionalized. Immobilizing single cells precisely on MEMS sensor sites is a requirement for many single cell analysis methods - reliability of cell analysis directly depends on the reliability of immobilization. The presented method uses magnetic forces to immobilize cells labeled with superparamagnetic beads on MEMS scale sites of interest. It can be coupled with many cell analysis techniques to form MEMS lab-on-a-chip devices due to the simple fabrication setup and principle of operation. The performance of the device was demonstrated with unbound superparamagnetic beads as well as with human breast tumor cells from the cell line MCF-7. Immobilization percentage and area were 95.2% and 1000 μm2, respectively, for superparamagnetic beads. MCF-7 cells labeled with superparamagnetic beads were immobilized at a percentage of 69.2% with the average distance from immobilized cells to micro-electromagnet being 50.8 μm. Thus, the presented method is a good candidate for integration with microfluidic sensing and analysis platforms.
KW - Cell immobilization
KW - circulating tumor cells
KW - microfluidics
KW - single cell analysis
KW - superparamagnetic beads
UR - http://www.scopus.com/inward/record.url?scp=84991517806&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991517806&partnerID=8YFLogxK
U2 - 10.1109/JSEN.2016.2600944
DO - 10.1109/JSEN.2016.2600944
M3 - Article
VL - 16
SP - 7572
EP - 7580
JO - IEEE Sensors Journal
JF - IEEE Sensors Journal
SN - 1530-437X
IS - 21
M1 - 7544558
ER -