Abstract
The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.
| Original language | English (US) |
|---|---|
| Article number | eaat0496 |
| Journal | Science advances |
| Volume | 4 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 23 2018 |
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Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy. / Fernandes, Jill N.; Dos Santos, Lílha M.B.; Chouin-Carneiro, Thaís; Pavan, Márcio G.; Garcia, Gabriela A.; David, Mariana R.; Beier, John C; Dowell, Floyd E.; Maciel-de-Freitas, Rafael; Sikulu-Lord, Maggy T.
In: Science advances, Vol. 4, No. 5, eaat0496, 23.05.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy
AU - Fernandes, Jill N.
AU - Dos Santos, Lílha M.B.
AU - Chouin-Carneiro, Thaís
AU - Pavan, Márcio G.
AU - Garcia, Gabriela A.
AU - David, Mariana R.
AU - Beier, John C
AU - Dowell, Floyd E.
AU - Maciel-de-Freitas, Rafael
AU - Sikulu-Lord, Maggy T.
PY - 2018/5/23
Y1 - 2018/5/23
N2 - The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.
AB - The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.
UR - http://www.scopus.com/inward/record.url?scp=85047482918&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047482918&partnerID=8YFLogxK
U2 - 10.1126/sciadv.aat0496
DO - 10.1126/sciadv.aat0496
M3 - Article
C2 - 29806030
AN - SCOPUS:85047482918
VL - 4
JO - Science advances
JF - Science advances
SN - 2375-2548
IS - 5
M1 - eaat0496
ER -