Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy

Jill N. Fernandes; Lílha M.B. Dos Santos; Thaís Chouin-Carneiro; Márcio G. Pavan; Gabriela A. Garcia; Mariana R. David; John C. Beier; Floyd E. Dowell; Rafael Maciel-de-Freitas; Maggy T. Sikulu-Lord

doi:10.1126/sciadv.aat0496

Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy

Jill N. Fernandes, Lílha M.B. Dos Santos, Thaís Chouin-Carneiro, Márcio G. Pavan, Gabriela A. Garcia, Mariana R. David, John C. Beier, Floyd E. Dowell, Rafael Maciel-de-Freitas, Maggy T. Sikulu-Lord

Public Health Sciences

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.

Original language	English (US)
Article number	eaat0496
Journal	Science Advances
Volume	4
Issue number	5
DOIs	https://doi.org/10.1126/sciadv.aat0496
State	Published - May 23 2018

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Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy. / Fernandes, Jill N.; Dos Santos, Lílha M.B.; Chouin-Carneiro, Thaís; Pavan, Márcio G.; Garcia, Gabriela A.; David, Mariana R.; Beier, John C.; Dowell, Floyd E.; Maciel-de-Freitas, Rafael; Sikulu-Lord, Maggy T.

In: Science Advances, Vol. 4, No. 5, eaat0496, 23.05.2018.

Research output: Contribution to journal › Article › peer-review

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abstract = "The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.",

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