Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy

Jill N. Fernandes, Lílha M.B. Dos Santos, Thaís Chouin-Carneiro, Márcio G. Pavan, Gabriela A. Garcia, Mariana R. David, John C Beier, Floyd E. Dowell, Rafael Maciel-de-Freitas, Maggy T. Sikulu-Lord

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.

Original languageEnglish (US)
Article numbereaat0496
JournalScience advances
Volume4
Issue number5
DOIs
StatePublished - May 23 2018

Fingerprint

Near-Infrared Spectroscopy
Aedes
Culicidae
Arboviruses
Reverse Transcription
Polymerase Chain Reaction
Disease Outbreaks
Thorax
Head
Light
Costs and Cost Analysis
Zika Virus

ASJC Scopus subject areas

  • General

Cite this

Fernandes, J. N., Dos Santos, L. M. B., Chouin-Carneiro, T., Pavan, M. G., Garcia, G. A., David, M. R., ... Sikulu-Lord, M. T. (2018). Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy. Science advances, 4(5), [eaat0496]. https://doi.org/10.1126/sciadv.aat0496

Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy. / Fernandes, Jill N.; Dos Santos, Lílha M.B.; Chouin-Carneiro, Thaís; Pavan, Márcio G.; Garcia, Gabriela A.; David, Mariana R.; Beier, John C; Dowell, Floyd E.; Maciel-de-Freitas, Rafael; Sikulu-Lord, Maggy T.

In: Science advances, Vol. 4, No. 5, eaat0496, 23.05.2018.

Research output: Contribution to journalArticle

Fernandes, JN, Dos Santos, LMB, Chouin-Carneiro, T, Pavan, MG, Garcia, GA, David, MR, Beier, JC, Dowell, FE, Maciel-de-Freitas, R & Sikulu-Lord, MT 2018, 'Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy', Science advances, vol. 4, no. 5, eaat0496. https://doi.org/10.1126/sciadv.aat0496
Fernandes JN, Dos Santos LMB, Chouin-Carneiro T, Pavan MG, Garcia GA, David MR et al. Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy. Science advances. 2018 May 23;4(5). eaat0496. https://doi.org/10.1126/sciadv.aat0496
Fernandes, Jill N. ; Dos Santos, Lílha M.B. ; Chouin-Carneiro, Thaís ; Pavan, Márcio G. ; Garcia, Gabriela A. ; David, Mariana R. ; Beier, John C ; Dowell, Floyd E. ; Maciel-de-Freitas, Rafael ; Sikulu-Lord, Maggy T. / Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy. In: Science advances. 2018 ; Vol. 4, No. 5.
@article{d6c3646c8fdc4f69b37fb7fa766e52ab,
title = "Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy",
abstract = "The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3{\%} relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.",
author = "Fernandes, {Jill N.} and {Dos Santos}, {L{\'i}lha M.B.} and Tha{\'i}s Chouin-Carneiro and Pavan, {M{\'a}rcio G.} and Garcia, {Gabriela A.} and David, {Mariana R.} and Beier, {John C} and Dowell, {Floyd E.} and Rafael Maciel-de-Freitas and Sikulu-Lord, {Maggy T.}",
year = "2018",
month = "5",
day = "23",
doi = "10.1126/sciadv.aat0496",
language = "English (US)",
volume = "4",
journal = "Science advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "5",

}

TY - JOUR

T1 - Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy

AU - Fernandes, Jill N.

AU - Dos Santos, Lílha M.B.

AU - Chouin-Carneiro, Thaís

AU - Pavan, Márcio G.

AU - Garcia, Gabriela A.

AU - David, Mariana R.

AU - Beier, John C

AU - Dowell, Floyd E.

AU - Maciel-de-Freitas, Rafael

AU - Sikulu-Lord, Maggy T.

PY - 2018/5/23

Y1 - 2018/5/23

N2 - The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.

AB - The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.

UR - http://www.scopus.com/inward/record.url?scp=85047482918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047482918&partnerID=8YFLogxK

U2 - 10.1126/sciadv.aat0496

DO - 10.1126/sciadv.aat0496

M3 - Article

C2 - 29806030

AN - SCOPUS:85047482918

VL - 4

JO - Science advances

JF - Science advances

SN - 2375-2548

IS - 5

M1 - eaat0496

ER -