Rapid, label-free, electrical whole blood bioassay based on nanobiosensor systems

Hsiao Kang Chang, Fumiaki N. Ishikawa, Rui Zhang, Ram Datar, Richard J. Cote, Mark E. Thompson, Chongwu Zhou

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Biomarker detection based on nanowire biosensors has attracted a significant amount of research effort in recent years. However, only very limited research work has been directed toward biomarker detection directly from physiological fluids mainly because of challenges caused by the complexity of media. This limitation significantly reduces the practical impact generated by the aforementioned nanobiosensors. In this study, we demonstrate an In 2O 3 nanowire-based biosensing system that is capable of performing rapid, label-free, electrical detection of cancer biomarkers directly from human whole blood collected by a finger prick. Passivating the nanowire surface successfully blocked the signal induced by nonspecific binding when performing active measurement in whole blood. Passivated devices showed markedly smaller signals induced by nonspecific binding of proteins and other biomaterials in serum and higher sensitivity to target biomarkers than bare devices. The detection limit of passivated sensors for biomarkers in whole blood was similar to the detection limit for the same analyte in purified buffer solutions at the same ionic strength, suggesting minimal decrease in device performance in the complex media. We then demonstrated detection of multiple cancer biomarkers with high reliability at clinically meaningful concentrations from whole blood collected by a finger prick using this sensing system.

Original languageEnglish (US)
Pages (from-to)9883-9891
Number of pages9
JournalACS Nano
Volume5
Issue number12
DOIs
StatePublished - Dec 27 2011

Keywords

  • biosensor
  • cancer biomarker detection
  • nanowire
  • whole blood detection

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

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  • Cite this

    Chang, H. K., Ishikawa, F. N., Zhang, R., Datar, R., Cote, R. J., Thompson, M. E., & Zhou, C. (2011). Rapid, label-free, electrical whole blood bioassay based on nanobiosensor systems. ACS Nano, 5(12), 9883-9891. https://doi.org/10.1021/nn2035796