Rapid induction of alkaline phosphatase activity by retinoic acid

David H. Reese; Gilbert J. Fiorentino; Alice J. Claflin; Theodore I. Malinin; Victor A. Politano

doi:10.1016/0006-291X(81)91523-0

Rapid induction of alkaline phosphatase activity by retinoic acid

David H. Reese, Gilbert J. Fiorentino, Alice J. Claflin, Theodore I. Malinin, Victor A. Politano

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35 Scopus citations

Abstract

Retinoic acid (vitamin A acid) increased alkaline phosphatase activity in cultured cells derived from both normal rat prostate and the Dunning R-3327 transplantable prostatic adenocarcinoma. Retinoic acid was found to be 3-4-fold more effective as an inducer of enzyme activity than retinol or retinal. In one rapidly-growing cell line (UMS-1541Q) which has a barely-detectable level of enzyme activity in the uninduced state, increased activity could be detected as early as 3-4 hours after the addition of 10μM retinoic acid. This increase was totally blocked by actinomycin D and cycloheximide. The demonstrated rapid inducibility of alkaline phosphatase activity provides a specific marker for the action of retinoic acid at the molecular level.

Original language	English (US)
Pages (from-to)	315-321
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	102
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(81)91523-0
State	Published - Sep 16 1981

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Reese, D. H., Fiorentino, G. J., Claflin, A. J., Malinin, T. I., & Politano, V. A. (1981). Rapid induction of alkaline phosphatase activity by retinoic acid. Biochemical and biophysical research communications, 102(1), 315-321. https://doi.org/10.1016/0006-291X(81)91523-0

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AU - Politano, Victor A.

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AB - Retinoic acid (vitamin A acid) increased alkaline phosphatase activity in cultured cells derived from both normal rat prostate and the Dunning R-3327 transplantable prostatic adenocarcinoma. Retinoic acid was found to be 3-4-fold more effective as an inducer of enzyme activity than retinol or retinal. In one rapidly-growing cell line (UMS-1541Q) which has a barely-detectable level of enzyme activity in the uninduced state, increased activity could be detected as early as 3-4 hours after the addition of 10μM retinoic acid. This increase was totally blocked by actinomycin D and cycloheximide. The demonstrated rapid inducibility of alkaline phosphatase activity provides a specific marker for the action of retinoic acid at the molecular level.

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