Abstract
Retinoic acid (vitamin A acid) increased alkaline phosphatase activity in cultured cells derived from both normal rat prostate and the Dunning R-3327 transplantable prostatic adenocarcinoma. Retinoic acid was found to be 3-4-fold more effective as an inducer of enzyme activity than retinol or retinal. In one rapidly-growing cell line (UMS-1541Q) which has a barely-detectable level of enzyme activity in the uninduced state, increased activity could be detected as early as 3-4 hours after the addition of 10μM retinoic acid. This increase was totally blocked by actinomycin D and cycloheximide. The demonstrated rapid inducibility of alkaline phosphatase activity provides a specific marker for the action of retinoic acid at the molecular level.
| Original language | English |
|---|---|
| Pages (from-to) | 315-321 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 102 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 16 1981 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Rapid induction of alkaline phosphatase activity by retinoic acid. / Reese, David H.; Fiorentino, Gilbert J.; Claflin, Alice J.; Malinin, Theodore; Politano, Victor A.
In: Biochemical and Biophysical Research Communications, Vol. 102, No. 1, 16.09.1981, p. 315-321.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid induction of alkaline phosphatase activity by retinoic acid
AU - Reese, David H.
AU - Fiorentino, Gilbert J.
AU - Claflin, Alice J.
AU - Malinin, Theodore
AU - Politano, Victor A.
PY - 1981/9/16
Y1 - 1981/9/16
N2 - Retinoic acid (vitamin A acid) increased alkaline phosphatase activity in cultured cells derived from both normal rat prostate and the Dunning R-3327 transplantable prostatic adenocarcinoma. Retinoic acid was found to be 3-4-fold more effective as an inducer of enzyme activity than retinol or retinal. In one rapidly-growing cell line (UMS-1541Q) which has a barely-detectable level of enzyme activity in the uninduced state, increased activity could be detected as early as 3-4 hours after the addition of 10μM retinoic acid. This increase was totally blocked by actinomycin D and cycloheximide. The demonstrated rapid inducibility of alkaline phosphatase activity provides a specific marker for the action of retinoic acid at the molecular level.
AB - Retinoic acid (vitamin A acid) increased alkaline phosphatase activity in cultured cells derived from both normal rat prostate and the Dunning R-3327 transplantable prostatic adenocarcinoma. Retinoic acid was found to be 3-4-fold more effective as an inducer of enzyme activity than retinol or retinal. In one rapidly-growing cell line (UMS-1541Q) which has a barely-detectable level of enzyme activity in the uninduced state, increased activity could be detected as early as 3-4 hours after the addition of 10μM retinoic acid. This increase was totally blocked by actinomycin D and cycloheximide. The demonstrated rapid inducibility of alkaline phosphatase activity provides a specific marker for the action of retinoic acid at the molecular level.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019782746&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(81)91523-0
DO - 10.1016/0006-291X(81)91523-0
M3 - Article
VL - 102
SP - 315
EP - 321
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -