This chapter studies cellular resistance to cancer chemotherapeutic agents, such as alkaloids, and antibiotics is related to their rapid efflux from the intracellular environment. Analytical methods, such as high-pressure liquid chromatography and spectrofluorometry, are used for the monitoring of cellular drug retention and efflux. Laser flow cytometry (FCM) offers a unique tool for the monitoring of fluorescent antitumor drug retention and its modulation in tumor cells. Besides its rapidity, the laser FCM method identifies the heterogeneity of drug retention and allows for the sorting of subpopulations for further biochemical or morphological characterization. Anthracyclines, such as doxorubicin and daunomycin, are important cancer chemotherapeutic agents. This method is used for the rapid monitoring of anthracycline transport and retention in drug-resistant and sensitive tumor cells. Cellular resistance to some of the clinically important anthracyclines has been suggested to be due to rapid drug efflux. Thus, drugs that block anthracycline efflux and thereby enhance retention can reduce cellular resistance to anthracyclines. The use of efflux blockers has been advocated for the chemotherapy of human malignancies.
ASJC Scopus subject areas
- Cell Biology