Rapid Complementation Assay for Anti-HIV-1 Drug Screening and Analysis of Envelope Protein Function

Paul Schiller, Rebeca Geffin, Richard Voellmy

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A complementation assay is described that can be used with relative safety to quantitate rapidly inhibitory effects of potential anti-HIV-1 drugs on virtually any stage of the HIV-1 life cycle by measurements of chloramphenicol acetyltransferase (CAT) activity. Of particular interest is that this system is also capable of detecting inhibition of the viral trans- activator Rev, an important potential target for drug intervention. Other applications of the system may include studies to identify domains of the envelope glycoprotein that determine infectivity and tropism or that define epitopes recognized by neutralization antibodies.

Original languageEnglish (US)
Pages (from-to)1723-1731
Number of pages9
JournalAIDS research and human retroviruses
Volume8
Issue number9
DOIs
StatePublished - Sep 1992

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ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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