Rapid communication: Inhibitory effect of a two day fast on reactive oxygen species (ROS) generation by leucocytes and plasma ortho-tyrosine and meta-tyrosine concentrations

P. Dandona, P. Mohanty, W. Hamouda, H. Ghanim, A. Aljada, R. Garg, V. Kumar

Research output: Contribution to journalArticlepeer-review

76 Scopus citations

Abstract

Since glucose intake acutely increases reactive oxygen species (ROS) generation by polymorphonuclear leucocytes (PMN) and mononuclear cells (MNC), we have now investigated whether a fast over a period of 48h reduces ROS generation by these cells. Eight normal subjects were fasted for 48h. Blood samples were obtained at 0, 24h and 48h. ROS generation by PMN fell significantly at 24h (66.1 ± 19.5 % of basal) and further at 48h (45.9 ± 23.0 % of basal; p<0.001). ROS generation by MNC fell to 62.4 ± 16.5 % at 24h and by 48.4 ± 16.5 % (p<0.001) by 48h. The level of p47phox subunit, an index of NADPH oxidase, the enzyme converting molecular oxygen to superoxide (O2-) radical, also fell in parallel Plasma o-tyrosine/phenylalanine ratio fell significantly from 0.326 ± 0.053 mmol/mol to 0.303 ± 0.055 mmol/mol at 48h and m-tyrosine/phenylalanine ratio fell from 0.363 ± 0.063 mmol/mol to 0.340 ± 0.064 mmol/mol (p<0.05). Thus, a 48h fast may reduce ROS generation, total oxidative load and oxidative damage to amino acids.

Original languageEnglish (US)
Pages (from-to)2899-2902
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume86
Issue number6
DOIs
StatePublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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