Rapid chemical sampling of endogenous species from brain slice preparations

Ramin Shiekhattar, Elizabeth G. Bibbs, Richard L. Schowen, Ralph N. Adams

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A very simple method of chemical sampling from the surface of brain slices is described. This procedure utilizing micropipets involves no sample dilution, and thus has very high sensitivity. Basal and stimulated concentrations of neurotransmitters and their metabolites are sampled from a thin fluid layer on the slice surface and appear to be in mobile equilibrium with the brain slice extracellular fluid levels. Rapid sampling allows one to follow the time course of stimulated release and reuptake phenomena.

Original languageEnglish (US)
Pages (from-to)189-194
Number of pages6
JournalNeuroscience Letters
Volume111
Issue number1-2
DOIs
StatePublished - Mar 26 1990
Externally publishedYes

Fingerprint

Extracellular Fluid
Brain
Neurotransmitter Agents

Keywords

  • Brain slice
  • Neurotransmitter release
  • Rapid chemical sampling

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Rapid chemical sampling of endogenous species from brain slice preparations. / Shiekhattar, Ramin; Bibbs, Elizabeth G.; Schowen, Richard L.; Adams, Ralph N.

In: Neuroscience Letters, Vol. 111, No. 1-2, 26.03.1990, p. 189-194.

Research output: Contribution to journalArticle

Shiekhattar, Ramin ; Bibbs, Elizabeth G. ; Schowen, Richard L. ; Adams, Ralph N. / Rapid chemical sampling of endogenous species from brain slice preparations. In: Neuroscience Letters. 1990 ; Vol. 111, No. 1-2. pp. 189-194.
@article{b7a7c2c9990c4fea95ba0464f630f3ef,
title = "Rapid chemical sampling of endogenous species from brain slice preparations",
abstract = "A very simple method of chemical sampling from the surface of brain slices is described. This procedure utilizing micropipets involves no sample dilution, and thus has very high sensitivity. Basal and stimulated concentrations of neurotransmitters and their metabolites are sampled from a thin fluid layer on the slice surface and appear to be in mobile equilibrium with the brain slice extracellular fluid levels. Rapid sampling allows one to follow the time course of stimulated release and reuptake phenomena.",
keywords = "Brain slice, Neurotransmitter release, Rapid chemical sampling",
author = "Ramin Shiekhattar and Bibbs, {Elizabeth G.} and Schowen, {Richard L.} and Adams, {Ralph N.}",
year = "1990",
month = "3",
day = "26",
doi = "10.1016/0304-3940(90)90366-H",
language = "English (US)",
volume = "111",
pages = "189--194",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - Rapid chemical sampling of endogenous species from brain slice preparations

AU - Shiekhattar, Ramin

AU - Bibbs, Elizabeth G.

AU - Schowen, Richard L.

AU - Adams, Ralph N.

PY - 1990/3/26

Y1 - 1990/3/26

N2 - A very simple method of chemical sampling from the surface of brain slices is described. This procedure utilizing micropipets involves no sample dilution, and thus has very high sensitivity. Basal and stimulated concentrations of neurotransmitters and their metabolites are sampled from a thin fluid layer on the slice surface and appear to be in mobile equilibrium with the brain slice extracellular fluid levels. Rapid sampling allows one to follow the time course of stimulated release and reuptake phenomena.

AB - A very simple method of chemical sampling from the surface of brain slices is described. This procedure utilizing micropipets involves no sample dilution, and thus has very high sensitivity. Basal and stimulated concentrations of neurotransmitters and their metabolites are sampled from a thin fluid layer on the slice surface and appear to be in mobile equilibrium with the brain slice extracellular fluid levels. Rapid sampling allows one to follow the time course of stimulated release and reuptake phenomena.

KW - Brain slice

KW - Neurotransmitter release

KW - Rapid chemical sampling

UR - http://www.scopus.com/inward/record.url?scp=0025266221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025266221&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(90)90366-H

DO - 10.1016/0304-3940(90)90366-H

M3 - Article

VL - 111

SP - 189

EP - 194

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1-2

ER -