Rapid array-based genomic characterization of a subtle structural abnormality: A patient with psychosis and der(18)t(5;18)(p14.1;p11.23)

Carolyn M. Drazinic, Adife G. Ercan-Sencicek, Laura M. Gault, Fuki M. Hisama, Mazin B. Qumsiyeh, Norma J. Nowak, Joseph F. Cubells, Matthew W. State

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Array-based copy number analysis has recently emerged as a rapid means of mapping complex and/or subtle chromosomal abnormalities. We have compared two such techniques, using bacterial artificial chromosome (BAG) and single nucleotide polymorphism (SNP) arrays in the evaluation of a 45-year-old woman with dysmorphic features, mental retardation, psychosis, and an unbalanced derivative chromosome 18, (46,XX, der(18)t(18;?)(p12;?)). Both array-based methods demonstrated that the additional material on chromosome 18 was of 5p origin. The 5p duplication mapped telomeric to 25.320 Mb (BAC array) and 25.607 Mb (SNP array), corresponding to the band 5p14.1. Both BAG and SNP arrays also showed a deletion involving chromosome 18p extending telomeric from 8.437 Mb (BAC array) and 8.352 Mb (SNP array), corresponding to the band 18p11.23. Molecular cytogenetic mapping using fluorescence in situ hybridization (FISH) supported the array findings and further refined the breakpoint regions, confirming that the BAG and SNP chips were both useful in this regard. Both case reports and linkage analyses have implicated these chromosomal intervals in psychosis. The array-based experiments were completed over the course of several days. While these methods do not eliminate the requirement for traditional fine-mapping, they provide an efficient approach to identifying the origin and extent of deleted and duplicated material in chromosomal rearrangements.

Original languageEnglish (US)
Pages (from-to)282-289
Number of pages8
JournalAmerican Journal of Medical Genetics
Volume134 A
Issue number3
DOIs
StatePublished - Apr 30 2005

Keywords

  • Chromosome abnormality
  • FISH
  • Mapping
  • Microarray
  • Monosomy 18p
  • Psychosis
  • Schizophrenia
  • Trisomy 5p
  • Unbalanced translocation

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Drazinic, C. M., Ercan-Sencicek, A. G., Gault, L. M., Hisama, F. M., Qumsiyeh, M. B., Nowak, N. J., Cubells, J. F., & State, M. W. (2005). Rapid array-based genomic characterization of a subtle structural abnormality: A patient with psychosis and der(18)t(5;18)(p14.1;p11.23). American Journal of Medical Genetics, 134 A(3), 282-289. https://doi.org/10.1002/ajmg.a.30616