Rapid accumulation of Akt in mitochondria following phosphatidylinositol 3-kinase activation

Gautam N. Bijur, Richard S. Jope

Research output: Contribution to journalArticle

246 Scopus citations

Abstract

We describe here a new component of the phosphatidylinositol 3-kinase/Akt signaling pathway that directly impacts mitochondria. Akt (protein kinase B) was shown for the first time to be localized in mitochondria, where it was found to reside in the matrix and the inner and outer membranes, and the level of mitochondrial Akt was very dynamically regulated. Stimulation of a variety of cell types with insulin-like growth factor-1, insulin, or stress (induced by heat shock), induced translocation of Akt to the mitochondria within only several minutes of stimulation, causing increases of nearly eight- to 12-fold, and the mitochondrial Akt was in its phosphorylated, active state. Two mitochondrial proteins were identified to be phosphorylated following stimulation of mitochondrial Akt, the β-subunit of ATP synthase and glycogen synthase kinase-3β. The finding that mitochondrial glycogen synthase kinase-3β was rapidly and substantially modified by Ser9 phosphorylation, which inhibits its activity, following translocation of Akt to the mitochondria is the first evidence for a regulatory mechanism affecting mitochondrial glycogen synthase kinase-3β. These results demonstrate that signals emanating from plasma membrane receptors or generated by stress rapidly modulate Akt and glycogen synthase kinase-3β in mitochondria.

Original languageEnglish (US)
Pages (from-to)1427-1435
Number of pages9
JournalJournal of neurochemistry
Volume87
Issue number6
DOIs
StatePublished - Dec 2003
Externally publishedYes

Keywords

  • Akt
  • ATP synthase
  • Glycogen synthase kinase-3β
  • Insulin-like growth factor-1
  • Mitochondria
  • Phosphatidylinositol 3-kinase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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