RAGE binds C1q and enhances C1q-mediated phagocytosis

Wanchao Ma, Vivek Rai, Barry Hudson, Fei Song, Ann Marie Schmidt, Gaetano R. Barile

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

RAGE, the multiligand receptor of the immunoglobulin superfamily of cell surface molecules, is implicated in innate and adaptive immunity. Complement component C1q serves roles in complement activation and antibody-independent opsonization. Using soluble forms of RAGE (sRAGE) and RAGE-expressing cells, we determined that RAGE is a native C1q globular domain receptor. Direct C1q-sRAGE interaction was demonstrated with surface plasmon resonance (SPR), with minimum K d 5.6μM, and stronger binding affinity seen in ELISA-like experiments involving multivalent binding. Pull-down experiments suggested formation of a receptor complex of RAGE and Mac-1 to further enhance affinity for C1q. C1q induced U937 cell adhesion and phagocytosis was inhibited by antibodies to RAGE or Mac-1. These data link C1q and RAGE to the recruitment of leukocytes and phagocytosis of C1q-coated material.

Original languageEnglish
Pages (from-to)72-82
Number of pages11
JournalCellular Immunology
Volume274
Issue number1-2
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

Fingerprint

Phagocytosis
Complement C1q
Cytophagocytosis
U937 Cells
Surface Plasmon Resonance
Antibodies
Complement Activation
Adaptive Immunity
Innate Immunity
Cell Adhesion
Immunoglobulins
Leukocytes
Enzyme-Linked Immunosorbent Assay

Keywords

  • Adhesion molecules
  • C1q
  • Complement
  • Efferocytosis
  • Macrophages
  • Monocytes
  • Phagocytosis
  • RAGE

ASJC Scopus subject areas

  • Immunology

Cite this

Ma, W., Rai, V., Hudson, B., Song, F., Schmidt, A. M., & Barile, G. R. (2012). RAGE binds C1q and enhances C1q-mediated phagocytosis. Cellular Immunology, 274(1-2), 72-82. https://doi.org/10.1016/j.cellimm.2012.02.001

RAGE binds C1q and enhances C1q-mediated phagocytosis. / Ma, Wanchao; Rai, Vivek; Hudson, Barry; Song, Fei; Schmidt, Ann Marie; Barile, Gaetano R.

In: Cellular Immunology, Vol. 274, No. 1-2, 01.03.2012, p. 72-82.

Research output: Contribution to journalArticle

Ma, W, Rai, V, Hudson, B, Song, F, Schmidt, AM & Barile, GR 2012, 'RAGE binds C1q and enhances C1q-mediated phagocytosis', Cellular Immunology, vol. 274, no. 1-2, pp. 72-82. https://doi.org/10.1016/j.cellimm.2012.02.001
Ma, Wanchao ; Rai, Vivek ; Hudson, Barry ; Song, Fei ; Schmidt, Ann Marie ; Barile, Gaetano R. / RAGE binds C1q and enhances C1q-mediated phagocytosis. In: Cellular Immunology. 2012 ; Vol. 274, No. 1-2. pp. 72-82.
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