Radioresistance in murine solid tumors induced by interleukin-1

Paul G Braunschweiger, Vathsala Basrur, Octavio Santos, Anthony Adessa, Pavel Houdek, Arnold Markoe

Research output: Contribution to journalArticle

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Abstract

Interleukin-1 (IL-1) has radioprotective activity in hematopoietic lineages and in other normal cell renewal systems, but little is known about the effects of IL-1α on the radiosensitivity of tumor cell populations. The present studies were conducted to investigate the effects of IL-1α on the radiosensitivity of clonogenic cells in RIF-1 and SCC-7 tumors. Radioresistance was detected within 2-4 h after administration of IL-1α (0.5 μg/mouse, ip) and characterized by increases in D(o), D(q), α/β and SF2. This radioresistance was similar to that seen in tumors rendered totally hypoxic before X irradiation. Tirapazamine, a hypoxic cell cytotoxin, and IL- 1α had synergistic schedule-dependent antitumor activity in vivo, suggesting that IL-1-induced radioresistance in vivo is due to hypoxia. Radioresistance induced by IL-1α was transient, and the data suggested reoxygenation within 12 h. In vitro, IL-1α had no direct effect on the radiosensitivity of SCC-7 cells in tissue culture under aerobic conditions. However, an increase in D(o), α/β and SF2 was seen in clonogenic tumor cells from primary cultures treated with IL-1α under aerobic conditions. Superoxide dismutase and catalase prevented the induction of radioresistance by IL-1α in vitro, suggesting that oxidative responses from tumor macrophages after administration of IL-1α may be responsible for induced radioresistance by IL-1 in vitro. Although oxidant stress induced by IL-1 may play an important role in the activity of IL-1α both in vivo and in vitro in our models, the mechanisms by which such responses modulate tumor radiosensitivity in vivo and in vitro are likely quite different.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalRadiation Research
Volume145
Issue number2
DOIs
StatePublished - Feb 1 1996

Fingerprint

interleukins
radiation resistance
interleukin-1
Interleukin-1
tumors
neoplasms
mice
Neoplasms
radiation tolerance
Radiation Tolerance
tirapazamine
aerobic conditions
cells
catalase
cytotoxins
macrophages
inorganic peroxides
hypoxia
Primary Cell Culture
schedules

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Braunschweiger, P. G., Basrur, V., Santos, O., Adessa, A., Houdek, P., & Markoe, A. (1996). Radioresistance in murine solid tumors induced by interleukin-1. Radiation Research, 145(2), 150-156. https://doi.org/10.2307/3579169

Radioresistance in murine solid tumors induced by interleukin-1. / Braunschweiger, Paul G; Basrur, Vathsala; Santos, Octavio; Adessa, Anthony; Houdek, Pavel; Markoe, Arnold.

In: Radiation Research, Vol. 145, No. 2, 01.02.1996, p. 150-156.

Research output: Contribution to journalArticle

Braunschweiger, PG, Basrur, V, Santos, O, Adessa, A, Houdek, P & Markoe, A 1996, 'Radioresistance in murine solid tumors induced by interleukin-1', Radiation Research, vol. 145, no. 2, pp. 150-156. https://doi.org/10.2307/3579169
Braunschweiger PG, Basrur V, Santos O, Adessa A, Houdek P, Markoe A. Radioresistance in murine solid tumors induced by interleukin-1. Radiation Research. 1996 Feb 1;145(2):150-156. https://doi.org/10.2307/3579169
Braunschweiger, Paul G ; Basrur, Vathsala ; Santos, Octavio ; Adessa, Anthony ; Houdek, Pavel ; Markoe, Arnold. / Radioresistance in murine solid tumors induced by interleukin-1. In: Radiation Research. 1996 ; Vol. 145, No. 2. pp. 150-156.
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