Radioimmunoassay of DSIP-like material in rat brain

Abba J. Kastin; Cheryl Nissen; Andrew V. Schally; David H. Coy

doi:10.1016/0361-9230(78)90019-9

Radioimmunoassay of DSIP-like material in rat brain

Abba J. Kastin, Cheryl Nissen, Andrew V. Schally, David H. Coy

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53 Scopus citations

Abstract

KASTIN, A. J., C. NISSEN, A. V. SCHALLY AND D. H. COY. Radioimmunoassay of DSIP-like material in rat brain. BRAIN RES. BULL. 3(6) 691-695, 1978.-Antibodies generated in a rabbit by immunization with synthetic delta-sleep inducing peptide (DSIP) showed no cross-reactivity with 19 naturally-occurring peptides or analogues and were used to establish a radioimmunoassay. Since DSIP is not readily iodinated by conventional methods, N-Tyr-DSIP was synthesized to prepare the tracer; the dose-response curve for N-Tyr-DSIP was exactly parallel to that for DSIP. With this assay, DSIP-like immunoreactivity was detected in the brain of rats. The highest brain values were found in the thalamus (11.9 ± 1.3 pg/mg). The widespread presence of DSIP-like material throughout the body as well as the shared amino acid sequences of DSIP with other proteins suggest caution in defining the material being measured by this new assay. Nevertheless, the demonstration of DSIP-like activity in brain tissue and elsewhere warrants consideration of functions in addition to a possible role in sleep.

Original language	English (US)
Pages (from-to)	691-695
Number of pages	5
Journal	Brain Research Bulletin
Volume	3
Issue number	6
DOIs	https://doi.org/10.1016/0361-9230(78)90019-9
State	Published - Jan 1 1978
Externally published	Yes

Keywords

Brain
DSIP
Peptide
Radioimmunoassay
Sleep

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0361-9230(78)90019-9

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title = "Radioimmunoassay of DSIP-like material in rat brain",

abstract = "KASTIN, A. J., C. NISSEN, A. V. SCHALLY AND D. H. COY. Radioimmunoassay of DSIP-like material in rat brain. BRAIN RES. BULL. 3(6) 691-695, 1978.-Antibodies generated in a rabbit by immunization with synthetic delta-sleep inducing peptide (DSIP) showed no cross-reactivity with 19 naturally-occurring peptides or analogues and were used to establish a radioimmunoassay. Since DSIP is not readily iodinated by conventional methods, N-Tyr-DSIP was synthesized to prepare the tracer; the dose-response curve for N-Tyr-DSIP was exactly parallel to that for DSIP. With this assay, DSIP-like immunoreactivity was detected in the brain of rats. The highest brain values were found in the thalamus (11.9 ± 1.3 pg/mg). The widespread presence of DSIP-like material throughout the body as well as the shared amino acid sequences of DSIP with other proteins suggest caution in defining the material being measured by this new assay. Nevertheless, the demonstration of DSIP-like activity in brain tissue and elsewhere warrants consideration of functions in addition to a possible role in sleep.",

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author = "Kastin, {Abba J.} and Cheryl Nissen and Schally, {Andrew V.} and Coy, {David H.}",

year = "1978",

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doi = "10.1016/0361-9230(78)90019-9",

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T1 - Radioimmunoassay of DSIP-like material in rat brain

AU - Kastin, Abba J.

AU - Nissen, Cheryl

AU - Schally, Andrew V.

AU - Coy, David H.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - KASTIN, A. J., C. NISSEN, A. V. SCHALLY AND D. H. COY. Radioimmunoassay of DSIP-like material in rat brain. BRAIN RES. BULL. 3(6) 691-695, 1978.-Antibodies generated in a rabbit by immunization with synthetic delta-sleep inducing peptide (DSIP) showed no cross-reactivity with 19 naturally-occurring peptides or analogues and were used to establish a radioimmunoassay. Since DSIP is not readily iodinated by conventional methods, N-Tyr-DSIP was synthesized to prepare the tracer; the dose-response curve for N-Tyr-DSIP was exactly parallel to that for DSIP. With this assay, DSIP-like immunoreactivity was detected in the brain of rats. The highest brain values were found in the thalamus (11.9 ± 1.3 pg/mg). The widespread presence of DSIP-like material throughout the body as well as the shared amino acid sequences of DSIP with other proteins suggest caution in defining the material being measured by this new assay. Nevertheless, the demonstration of DSIP-like activity in brain tissue and elsewhere warrants consideration of functions in addition to a possible role in sleep.

AB - KASTIN, A. J., C. NISSEN, A. V. SCHALLY AND D. H. COY. Radioimmunoassay of DSIP-like material in rat brain. BRAIN RES. BULL. 3(6) 691-695, 1978.-Antibodies generated in a rabbit by immunization with synthetic delta-sleep inducing peptide (DSIP) showed no cross-reactivity with 19 naturally-occurring peptides or analogues and were used to establish a radioimmunoassay. Since DSIP is not readily iodinated by conventional methods, N-Tyr-DSIP was synthesized to prepare the tracer; the dose-response curve for N-Tyr-DSIP was exactly parallel to that for DSIP. With this assay, DSIP-like immunoreactivity was detected in the brain of rats. The highest brain values were found in the thalamus (11.9 ± 1.3 pg/mg). The widespread presence of DSIP-like material throughout the body as well as the shared amino acid sequences of DSIP with other proteins suggest caution in defining the material being measured by this new assay. Nevertheless, the demonstration of DSIP-like activity in brain tissue and elsewhere warrants consideration of functions in addition to a possible role in sleep.

KW - Brain

KW - DSIP

KW - Peptide

KW - Radioimmunoassay

KW - Sleep

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Radioimmunoassay of DSIP-like material in rat brain

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