Radioimmunoassay of DSIP-like material in rat brain

Abba J. Kastin, Cheryl Nissen, Andrew V. Schally, David H. Coy

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

KASTIN, A. J., C. NISSEN, A. V. SCHALLY AND D. H. COY. Radioimmunoassay of DSIP-like material in rat brain. BRAIN RES. BULL. 3(6) 691-695, 1978.-Antibodies generated in a rabbit by immunization with synthetic delta-sleep inducing peptide (DSIP) showed no cross-reactivity with 19 naturally-occurring peptides or analogues and were used to establish a radioimmunoassay. Since DSIP is not readily iodinated by conventional methods, N-Tyr-DSIP was synthesized to prepare the tracer; the dose-response curve for N-Tyr-DSIP was exactly parallel to that for DSIP. With this assay, DSIP-like immunoreactivity was detected in the brain of rats. The highest brain values were found in the thalamus (11.9 ± 1.3 pg/mg). The widespread presence of DSIP-like material throughout the body as well as the shared amino acid sequences of DSIP with other proteins suggest caution in defining the material being measured by this new assay. Nevertheless, the demonstration of DSIP-like activity in brain tissue and elsewhere warrants consideration of functions in addition to a possible role in sleep.

Original languageEnglish (US)
Pages (from-to)691-695
Number of pages5
JournalBrain Research Bulletin
Volume3
Issue number6
DOIs
StatePublished - Jan 1 1978
Externally publishedYes

Keywords

  • Brain
  • DSIP
  • Peptide
  • Radioimmunoassay
  • Sleep

ASJC Scopus subject areas

  • Neuroscience(all)

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