Abstract
We have developed a sensitive immunoassay for murine calcitonin which can measure the basal concentration of the hormone in peripheral plasma of rats as well as the calcitonin extracted from rat thyroid tissue. Human calcitonin was used for tracer, standard, and antibody production. Assay methods were devised to minimize artifacts which have been shown to spuriously influence immunoassay performance. The basal plasma concentration of calcitonin in rats was 6 to 75 pg. per milliliter. Infusions of calcium, pentagastrin, and PGE2 produced a 2- to 7-fold increase in plasma calcitonin. Elevated concentrations of calcitonin were demonstrated in tumor specimens obtained from the second and fourth generations of a transplanted rat medullary thyroid carcinoma. Our results demonstrate that calcitonin circulates in the peripheral plasma of rats in the basal state and that hormone concentrations change during functional tests of secretion. This assay will permit studies of calcitonin secretion in physiological and pathophysiological states in the rat.
| Original language | English |
|---|---|
| Pages (from-to) | 173-182 |
| Number of pages | 10 |
| Journal | The Journal of laboratory and clinical medicine |
| Volume | 88 |
| Issue number | 2 |
| State | Published - Jan 1 1976 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
- Pathology and Forensic Medicine
Cite this
Radioimmunoassay of calcitonin in plasma, normal thyroid, and medullary thyroid carcinoma of the rat. / Roos, B. A.; Deftos, L. J.
In: The Journal of laboratory and clinical medicine, Vol. 88, No. 2, 01.01.1976, p. 173-182.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Radioimmunoassay of calcitonin in plasma, normal thyroid, and medullary thyroid carcinoma of the rat
AU - Roos, B. A.
AU - Deftos, L. J.
PY - 1976/1/1
Y1 - 1976/1/1
N2 - We have developed a sensitive immunoassay for murine calcitonin which can measure the basal concentration of the hormone in peripheral plasma of rats as well as the calcitonin extracted from rat thyroid tissue. Human calcitonin was used for tracer, standard, and antibody production. Assay methods were devised to minimize artifacts which have been shown to spuriously influence immunoassay performance. The basal plasma concentration of calcitonin in rats was 6 to 75 pg. per milliliter. Infusions of calcium, pentagastrin, and PGE2 produced a 2- to 7-fold increase in plasma calcitonin. Elevated concentrations of calcitonin were demonstrated in tumor specimens obtained from the second and fourth generations of a transplanted rat medullary thyroid carcinoma. Our results demonstrate that calcitonin circulates in the peripheral plasma of rats in the basal state and that hormone concentrations change during functional tests of secretion. This assay will permit studies of calcitonin secretion in physiological and pathophysiological states in the rat.
AB - We have developed a sensitive immunoassay for murine calcitonin which can measure the basal concentration of the hormone in peripheral plasma of rats as well as the calcitonin extracted from rat thyroid tissue. Human calcitonin was used for tracer, standard, and antibody production. Assay methods were devised to minimize artifacts which have been shown to spuriously influence immunoassay performance. The basal plasma concentration of calcitonin in rats was 6 to 75 pg. per milliliter. Infusions of calcium, pentagastrin, and PGE2 produced a 2- to 7-fold increase in plasma calcitonin. Elevated concentrations of calcitonin were demonstrated in tumor specimens obtained from the second and fourth generations of a transplanted rat medullary thyroid carcinoma. Our results demonstrate that calcitonin circulates in the peripheral plasma of rats in the basal state and that hormone concentrations change during functional tests of secretion. This assay will permit studies of calcitonin secretion in physiological and pathophysiological states in the rat.
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UR - http://www.scopus.com/inward/citedby.url?scp=0017126257&partnerID=8YFLogxK
M3 - Article
C2 - 822106
AN - SCOPUS:0017126257
VL - 88
SP - 173
EP - 182
JO - Translational Research
JF - Translational Research
SN - 1931-5244
IS - 2
ER -