Radioimmunoassay of calcitonin in plasma, normal thyroid, and medullary thyroid carcinoma of the rat

B. A. Roos, L. J. Deftos

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We have developed a sensitive immunoassay for murine calcitonin which can measure the basal concentration of the hormone in peripheral plasma of rats as well as the calcitonin extracted from rat thyroid tissue. Human calcitonin was used for tracer, standard, and antibody production. Assay methods were devised to minimize artifacts which have been shown to spuriously influence immunoassay performance. The basal plasma concentration of calcitonin in rats was 6 to 75 pg. per milliliter. Infusions of calcium, pentagastrin, and PGE2 produced a 2- to 7-fold increase in plasma calcitonin. Elevated concentrations of calcitonin were demonstrated in tumor specimens obtained from the second and fourth generations of a transplanted rat medullary thyroid carcinoma. Our results demonstrate that calcitonin circulates in the peripheral plasma of rats in the basal state and that hormone concentrations change during functional tests of secretion. This assay will permit studies of calcitonin secretion in physiological and pathophysiological states in the rat.

Original languageEnglish
Pages (from-to)173-182
Number of pages10
JournalThe Journal of laboratory and clinical medicine
Volume88
Issue number2
StatePublished - Jan 1 1976
Externally publishedYes

Fingerprint

Calcitonin
Radioimmunoassay
Rats
Thyroid Gland
Plasmas
Immunoassay
Assays
Hormones
Pentagastrin
Medullary Thyroid cancer
Dinoprostone
Artifacts
Antibody Formation
Tumors
Tissue
Calcium
Antibodies

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Radioimmunoassay of calcitonin in plasma, normal thyroid, and medullary thyroid carcinoma of the rat. / Roos, B. A.; Deftos, L. J.

In: The Journal of laboratory and clinical medicine, Vol. 88, No. 2, 01.01.1976, p. 173-182.

Research output: Contribution to journalArticle

@article{408de10fde45481e8e8e413ce97b4667,
title = "Radioimmunoassay of calcitonin in plasma, normal thyroid, and medullary thyroid carcinoma of the rat",
abstract = "We have developed a sensitive immunoassay for murine calcitonin which can measure the basal concentration of the hormone in peripheral plasma of rats as well as the calcitonin extracted from rat thyroid tissue. Human calcitonin was used for tracer, standard, and antibody production. Assay methods were devised to minimize artifacts which have been shown to spuriously influence immunoassay performance. The basal plasma concentration of calcitonin in rats was 6 to 75 pg. per milliliter. Infusions of calcium, pentagastrin, and PGE2 produced a 2- to 7-fold increase in plasma calcitonin. Elevated concentrations of calcitonin were demonstrated in tumor specimens obtained from the second and fourth generations of a transplanted rat medullary thyroid carcinoma. Our results demonstrate that calcitonin circulates in the peripheral plasma of rats in the basal state and that hormone concentrations change during functional tests of secretion. This assay will permit studies of calcitonin secretion in physiological and pathophysiological states in the rat.",
author = "Roos, {B. A.} and Deftos, {L. J.}",
year = "1976",
month = "1",
day = "1",
language = "English",
volume = "88",
pages = "173--182",
journal = "Translational Research",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - Radioimmunoassay of calcitonin in plasma, normal thyroid, and medullary thyroid carcinoma of the rat

AU - Roos, B. A.

AU - Deftos, L. J.

PY - 1976/1/1

Y1 - 1976/1/1

N2 - We have developed a sensitive immunoassay for murine calcitonin which can measure the basal concentration of the hormone in peripheral plasma of rats as well as the calcitonin extracted from rat thyroid tissue. Human calcitonin was used for tracer, standard, and antibody production. Assay methods were devised to minimize artifacts which have been shown to spuriously influence immunoassay performance. The basal plasma concentration of calcitonin in rats was 6 to 75 pg. per milliliter. Infusions of calcium, pentagastrin, and PGE2 produced a 2- to 7-fold increase in plasma calcitonin. Elevated concentrations of calcitonin were demonstrated in tumor specimens obtained from the second and fourth generations of a transplanted rat medullary thyroid carcinoma. Our results demonstrate that calcitonin circulates in the peripheral plasma of rats in the basal state and that hormone concentrations change during functional tests of secretion. This assay will permit studies of calcitonin secretion in physiological and pathophysiological states in the rat.

AB - We have developed a sensitive immunoassay for murine calcitonin which can measure the basal concentration of the hormone in peripheral plasma of rats as well as the calcitonin extracted from rat thyroid tissue. Human calcitonin was used for tracer, standard, and antibody production. Assay methods were devised to minimize artifacts which have been shown to spuriously influence immunoassay performance. The basal plasma concentration of calcitonin in rats was 6 to 75 pg. per milliliter. Infusions of calcium, pentagastrin, and PGE2 produced a 2- to 7-fold increase in plasma calcitonin. Elevated concentrations of calcitonin were demonstrated in tumor specimens obtained from the second and fourth generations of a transplanted rat medullary thyroid carcinoma. Our results demonstrate that calcitonin circulates in the peripheral plasma of rats in the basal state and that hormone concentrations change during functional tests of secretion. This assay will permit studies of calcitonin secretion in physiological and pathophysiological states in the rat.

UR - http://www.scopus.com/inward/record.url?scp=0017126257&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017126257&partnerID=8YFLogxK

M3 - Article

C2 - 822106

AN - SCOPUS:0017126257

VL - 88

SP - 173

EP - 182

JO - Translational Research

JF - Translational Research

SN - 1931-5244

IS - 2

ER -