Radiographic and histologic effects of chondroitinase ABC on normal canine lumbar intervertebral disc

Thomas R. Fry, Jo Ann C. Eurell, L. Johnson, Mark D. Brown, John M. Losonsky, David J. Schaeffer

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Twelve dogs were divided into two equal groups and given lumbar intradiscal injections of 10, 50, or 100 U/ml of chondroitinase ABC reconstituted in sodium acetate buffer. Radiographs of the lumbar spine were made before and after surgery in both groups. Additional films were made at 5 days after surgery in Group I and at 7, 14, and 21 days after surgery in Group II. All spaces injected with 50 or 100 U/ml chondroitinase ABC demonstrated significant radiographic narrowing in both groups compared with uninjected control and buffer injected discs (P < 0.001). Discs injected with 10 U/ml of chondroitinase ABC showed increased narrowing over time from 7 to 21 days (P < 0.05). A zone of safranin O depletion was present in the ventral anulus fibrosus adjacent to the nucleus pulposus in all treated discs, indicating proteoglycan loss. All histologic effects of chondroitinase ABC were confined to intervertebral disc tissues. Chondroitinase ABC appears to be effective for chemonucleolysis in dogs.

Original languageEnglish (US)
Pages (from-to)816-819
Number of pages4
JournalSPINE
Volume16
Issue number7
DOIs
StatePublished - Jul 1991

Keywords

  • chemonucleolysis
  • chondroitinase ABC
  • histology
  • intervertebral disc
  • radiology

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Orthopedics and Sports Medicine

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