Radioautographic localization of radioactivity in rat brain after intraventricular or intracarotid injection of 3H-L-prolyl-L-leucyl glycinamide

Georges Pelletier, Fernand Labrie, Abba J. Kastin, David Coy, Andrew V Schally

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Tritiated L-prolyl-L-leucyl-glycinamide was injected into the carotid artery or lateral ventricle of rats and the radioactivity localized by radioautography. After intracarotid administration, the radioactivity labeled the meninges, ependymal cells bordering the ventricles, and cells of the choroid plexus, as well as the median eminence and subfornical organ and thus may have reached the cerebrospinal fluid. After intraventricular injection, specific localization of radioactivity was found in cells of the nucleus lateralis septi, nucleus medialis septi, putamen, globus pallidus, indusium griseum, hippocampus, corpus callosum, and meninges. The results after intracarotid injection suggest that L-prolyl-L-leucyl-glycinamide may cross the blood brain barrier and the results after intraventricular injection suggest sites of localization which might be correlated with the previously reported effects of the tripeptide on the brain.

Original languageEnglish
Pages (from-to)675-679
Number of pages5
JournalPharmacology, Biochemistry and Behavior
Volume3
Issue number4
DOIs
StatePublished - Jan 1 1975
Externally publishedYes

Fingerprint

MSH Release-Inhibiting Hormone
Radioactivity
Intraventricular Injections
Rats
Meninges
Brain
Injections
Subfornical Organ
Cerebrospinal fluid
Septal Nuclei
Median Eminence
Choroid Plexus
Globus Pallidus
Corpus Callosum
Lateral Ventricles
Putamen
Blood-Brain Barrier
Autoradiography
Cell Nucleus
Carotid Arteries

Keywords

  • Brain
  • Hormone
  • MIF
  • Peptide
  • Radioautography

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

Cite this

Radioautographic localization of radioactivity in rat brain after intraventricular or intracarotid injection of 3H-L-prolyl-L-leucyl glycinamide. / Pelletier, Georges; Labrie, Fernand; Kastin, Abba J.; Coy, David; Schally, Andrew V.

In: Pharmacology, Biochemistry and Behavior, Vol. 3, No. 4, 01.01.1975, p. 675-679.

Research output: Contribution to journalArticle

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AB - Tritiated L-prolyl-L-leucyl-glycinamide was injected into the carotid artery or lateral ventricle of rats and the radioactivity localized by radioautography. After intracarotid administration, the radioactivity labeled the meninges, ependymal cells bordering the ventricles, and cells of the choroid plexus, as well as the median eminence and subfornical organ and thus may have reached the cerebrospinal fluid. After intraventricular injection, specific localization of radioactivity was found in cells of the nucleus lateralis septi, nucleus medialis septi, putamen, globus pallidus, indusium griseum, hippocampus, corpus callosum, and meninges. The results after intracarotid injection suggest that L-prolyl-L-leucyl-glycinamide may cross the blood brain barrier and the results after intraventricular injection suggest sites of localization which might be correlated with the previously reported effects of the tripeptide on the brain.

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