Radiation treatment inhibits monocyte entry into the optic nerve head and prevents neuronal damage in a mouse model of glaucoma

Gareth R. Howell, Ileana Soto, Xianjun Zhu, Margaret Ryan, Danilo G. Macalinao, Gregory L. Sousa, Lura B. Caddle, Katharine H. MacNicoll, Jessica M. Barbay, Vittorio Porciatti, Michael G. Anderson, Richard S. Smith, Abbot F. Clark, Richard T. Libby, Simon W M John

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Glaucoma is a common ocular disorder that is a leading cause of blindness worldwide. It is characterized by the dysfunction and loss of retinal ganglion cells (RGCs). Although many studies have implicated various molecules in glaucoma, no mechanism has been shown to be responsible for the earliest detectable damage to RGCs and their axons in the optic nerve. Here, we show that the leukocyte transendothelial migration pathway is activated in the optic nerve head at the earliest stages of disease in an inherited mouse model of glaucoma. This resulted in proinflammatory monocytes entering the optic nerve prior to detectable neuronal damage. A 1-time x-ray treatment prevented monocyte entry and subsequent glaucomatous damage. A single x-ray treatment of an individual eye in young mice provided that eye with long-term protection from glaucoma but had no effect on the contralateral eye. Localized radiation treatment prevented detectable neuronal damage and dysfunction in treated eyes, despite the continued presence of other glaucomatous stresses and signaling pathways. Injection of endothelin-2, a damaging mediator produced by the monocytes, into irradiated eyes, combined with the other glaucomatous stresses, restored neural damage with a topography characteristic of glaucoma. Together, these data support a model of glaucomatous damage involving monocyte entry into the optic nerve.

Original languageEnglish
Pages (from-to)1246-1261
Number of pages16
JournalJournal of Clinical Investigation
Volume122
Issue number4
DOIs
StatePublished - Apr 2 2012

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Optic Disk
Glaucoma
Monocytes
Radiation
Optic Nerve
Retinal Ganglion Cells
Therapeutics
X-Rays
Endothelin-2
Transendothelial and Transepithelial Migration
Blindness
Axons
Leukocytes
Injections

ASJC Scopus subject areas

  • Medicine(all)

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Radiation treatment inhibits monocyte entry into the optic nerve head and prevents neuronal damage in a mouse model of glaucoma. / Howell, Gareth R.; Soto, Ileana; Zhu, Xianjun; Ryan, Margaret; Macalinao, Danilo G.; Sousa, Gregory L.; Caddle, Lura B.; MacNicoll, Katharine H.; Barbay, Jessica M.; Porciatti, Vittorio; Anderson, Michael G.; Smith, Richard S.; Clark, Abbot F.; Libby, Richard T.; John, Simon W M.

In: Journal of Clinical Investigation, Vol. 122, No. 4, 02.04.2012, p. 1246-1261.

Research output: Contribution to journalArticle

Howell, GR, Soto, I, Zhu, X, Ryan, M, Macalinao, DG, Sousa, GL, Caddle, LB, MacNicoll, KH, Barbay, JM, Porciatti, V, Anderson, MG, Smith, RS, Clark, AF, Libby, RT & John, SWM 2012, 'Radiation treatment inhibits monocyte entry into the optic nerve head and prevents neuronal damage in a mouse model of glaucoma', Journal of Clinical Investigation, vol. 122, no. 4, pp. 1246-1261. https://doi.org/10.1172/JCI61135
Howell, Gareth R. ; Soto, Ileana ; Zhu, Xianjun ; Ryan, Margaret ; Macalinao, Danilo G. ; Sousa, Gregory L. ; Caddle, Lura B. ; MacNicoll, Katharine H. ; Barbay, Jessica M. ; Porciatti, Vittorio ; Anderson, Michael G. ; Smith, Richard S. ; Clark, Abbot F. ; Libby, Richard T. ; John, Simon W M. / Radiation treatment inhibits monocyte entry into the optic nerve head and prevents neuronal damage in a mouse model of glaucoma. In: Journal of Clinical Investigation. 2012 ; Vol. 122, No. 4. pp. 1246-1261.
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