Rac2-deficient mice display perturbed T-cell distribution and chemotaxis, but only minor abnormalities in TH1 responses

Ben A. Croker, Emanuela Handman, John D. Hayball, Tracey M. Baldwin, Valentina Voigt, Leonie A. Cluse, Feng-Chun Yang, David A. Williams, Andrew W. Roberts

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The haematopoietic-specific RhoGTPase, Rac2, has been indirectly implicated in T-lymphocyte development and function, and as a pivotal regulator of T Helper 1 (TH1) responses. In other haematopoietic cells it regulates cytoskeletal rearrangement downstream of extracellular signals. Here we demonstrate that Rac2 deficiency results in an abnormal distribution of T lymphocytes in vivo and defects in T-lymphocyte migration and filamentous actin generation in response to chemoattractants in vitro. To investigate the requirement for Rac2 in IFN-γ production and TH1 responses in vivo, Rac2-deficient mice were challenged with Leishmania major and immunized with ovalbumin-expressing cytomegalovirus. Despite a minor skewing towards a TH2 phenotype, Rac2-deficient mice displayed no increased susceptibility to L. major infection. Cytotoxic T-lymphocyte responses to cytomegalovirus and ovalbumin were also normal. Although Rac2 is required for normal T-lymphocyte migration, its role in the generation of TH1 responses to infection in vivo is largely redundant.

Original languageEnglish (US)
Pages (from-to)231-240
Number of pages10
JournalImmunology and Cell Biology
Volume80
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

T-cells
Chemotaxis
T-Lymphocytes
Leishmania major
Ovalbumin
Cytomegalovirus
Chemotactic Factors
Cytotoxic T-Lymphocytes
Infection
Actins
Phenotype
Defects

Keywords

  • Chemotaxis
  • Rac2
  • T lymphocytes
  • T1/T2

ASJC Scopus subject areas

  • Immunology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Croker, B. A., Handman, E., Hayball, J. D., Baldwin, T. M., Voigt, V., Cluse, L. A., ... Roberts, A. W. (2002). Rac2-deficient mice display perturbed T-cell distribution and chemotaxis, but only minor abnormalities in TH1 responses. Immunology and Cell Biology, 80(3), 231-240. https://doi.org/10.1046/j.1440-1711.2002.01077.x

Rac2-deficient mice display perturbed T-cell distribution and chemotaxis, but only minor abnormalities in TH1 responses. / Croker, Ben A.; Handman, Emanuela; Hayball, John D.; Baldwin, Tracey M.; Voigt, Valentina; Cluse, Leonie A.; Yang, Feng-Chun; Williams, David A.; Roberts, Andrew W.

In: Immunology and Cell Biology, Vol. 80, No. 3, 2002, p. 231-240.

Research output: Contribution to journalArticle

Croker, BA, Handman, E, Hayball, JD, Baldwin, TM, Voigt, V, Cluse, LA, Yang, F-C, Williams, DA & Roberts, AW 2002, 'Rac2-deficient mice display perturbed T-cell distribution and chemotaxis, but only minor abnormalities in TH1 responses', Immunology and Cell Biology, vol. 80, no. 3, pp. 231-240. https://doi.org/10.1046/j.1440-1711.2002.01077.x
Croker, Ben A. ; Handman, Emanuela ; Hayball, John D. ; Baldwin, Tracey M. ; Voigt, Valentina ; Cluse, Leonie A. ; Yang, Feng-Chun ; Williams, David A. ; Roberts, Andrew W. / Rac2-deficient mice display perturbed T-cell distribution and chemotaxis, but only minor abnormalities in TH1 responses. In: Immunology and Cell Biology. 2002 ; Vol. 80, No. 3. pp. 231-240.
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