Rac1 is required for cell proliferation and G2/M progression

Kimberly A. Moore, Rachna Sethi, A. Masharn Doanes, Thomas M. Johnson, John B. Pracyk, Martha Kirby, Kaikobad Irani, Pascal J. Goldschmidt-Clermont, Toren Finkel

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.

Original languageEnglish (US)
Pages (from-to)17-20
Number of pages4
JournalBiochemical Journal
Volume326
Issue number1
DOIs
StatePublished - Aug 15 1997

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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