Rac1 is required for cell proliferation and G2/M progression

Kimberly A. Moore, Rachna Sethi, A. Masharn Doanes, Thomas M. Johnson, John B. Pracyk, Martha Kirby, Kaikobad Irani, Pascal Goldschmidt-Clermont, Toren Finkel

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.

Original languageEnglish
Pages (from-to)17-20
Number of pages4
JournalBiochemical Journal
Volume326
Issue number1
StatePublished - Aug 15 1997
Externally publishedYes

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Cell proliferation
Cells
Cell Proliferation
Cytostatic Agents
GTP-Binding Proteins
Gene transfer
Cell Cycle
Fibroblasts
Rats
Demonstrations
Growth
Infection
Genes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Moore, K. A., Sethi, R., Doanes, A. M., Johnson, T. M., Pracyk, J. B., Kirby, M., ... Finkel, T. (1997). Rac1 is required for cell proliferation and G2/M progression. Biochemical Journal, 326(1), 17-20.

Rac1 is required for cell proliferation and G2/M progression. / Moore, Kimberly A.; Sethi, Rachna; Doanes, A. Masharn; Johnson, Thomas M.; Pracyk, John B.; Kirby, Martha; Irani, Kaikobad; Goldschmidt-Clermont, Pascal; Finkel, Toren.

In: Biochemical Journal, Vol. 326, No. 1, 15.08.1997, p. 17-20.

Research output: Contribution to journalArticle

Moore, KA, Sethi, R, Doanes, AM, Johnson, TM, Pracyk, JB, Kirby, M, Irani, K, Goldschmidt-Clermont, P & Finkel, T 1997, 'Rac1 is required for cell proliferation and G2/M progression', Biochemical Journal, vol. 326, no. 1, pp. 17-20.
Moore KA, Sethi R, Doanes AM, Johnson TM, Pracyk JB, Kirby M et al. Rac1 is required for cell proliferation and G2/M progression. Biochemical Journal. 1997 Aug 15;326(1):17-20.
Moore, Kimberly A. ; Sethi, Rachna ; Doanes, A. Masharn ; Johnson, Thomas M. ; Pracyk, John B. ; Kirby, Martha ; Irani, Kaikobad ; Goldschmidt-Clermont, Pascal ; Finkel, Toren. / Rac1 is required for cell proliferation and G2/M progression. In: Biochemical Journal. 1997 ; Vol. 326, No. 1. pp. 17-20.
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