Rabbit trochlear model of osteochondral allograft transplantation

Nhat To, Shane Curtiss, Corey P. Neu, Christopher Salgado, Amir A. Jamali

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Allografting and autografting of osteochondral tissues is a promising strategy to treat articular cartilage lesions in damaged joints. We developed a new model of fresh osteochondral allografting using the entire rabbit trochlea. The objective of the current study was to demonstrate that this model would achieve reproducible graft-host healing and maintain normal articular cartilage histologic, immunolocalization, and biochemical characteristics after transplantation under diverse storage and transplantation conditions. New Zealand white (n = 8) and Dutch belted (n = 8) rabbits underwent a 2-stage transplantation operation using osteochondral grafts that had been stored for 2 or 4 wk. Trochlear grafts harvested from the left knee were transplanted to the right knee as either autografts or allografts. Grafts were fixed with 22-gauge steel wire or 3-0 nylon suture. Rabbits were euthanized for evaluation at 1, 2, 4, 6, and 12 wk after transplantation. All grafts that remained in vivo for at least 4 wk demonstrated 100% interface healing by microCT. Trabecular bridging was present at the host-graft interface starting at 2 wk after transplantation, with no significant difference in cartilage histology between the various groups. The combined histology scores indicated minimal evidence of osteoarthritis. Immunostaining revealed that superficial zone protein was localized at the surface of all transplants. The rabbit trochlear model met our criteria for a successful model in regard to the ease of the procedure, low rate of surgical complications, relatively large articular cartilage surface area, and amount of host-graft bone interface available for analysis.

Original languageEnglish
Pages (from-to)427-435
Number of pages9
JournalComparative Medicine
Volume61
Issue number5
StatePublished - Oct 1 2011

Fingerprint

allografting
Grafts
cartilage
Allografts
Transplantation
rabbits
Rabbits
Transplants
Cartilage
Transplantation (surgical)
knees
Dutch Belted (rabbit breed)
histology
Articular Cartilage
autografting
Histology
osteoarthritis
Homologous Transplantation
sutures
nylon

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

To, N., Curtiss, S., Neu, C. P., Salgado, C., & Jamali, A. A. (2011). Rabbit trochlear model of osteochondral allograft transplantation. Comparative Medicine, 61(5), 427-435.

Rabbit trochlear model of osteochondral allograft transplantation. / To, Nhat; Curtiss, Shane; Neu, Corey P.; Salgado, Christopher; Jamali, Amir A.

In: Comparative Medicine, Vol. 61, No. 5, 01.10.2011, p. 427-435.

Research output: Contribution to journalArticle

To, N, Curtiss, S, Neu, CP, Salgado, C & Jamali, AA 2011, 'Rabbit trochlear model of osteochondral allograft transplantation', Comparative Medicine, vol. 61, no. 5, pp. 427-435.
To N, Curtiss S, Neu CP, Salgado C, Jamali AA. Rabbit trochlear model of osteochondral allograft transplantation. Comparative Medicine. 2011 Oct 1;61(5):427-435.
To, Nhat ; Curtiss, Shane ; Neu, Corey P. ; Salgado, Christopher ; Jamali, Amir A. / Rabbit trochlear model of osteochondral allograft transplantation. In: Comparative Medicine. 2011 ; Vol. 61, No. 5. pp. 427-435.
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