Rabbit diaphragm: two types of fibres determined by calcium strontium activation and protein content.

P. E. Hoar, W. G. Kerrick

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Submaximum Ca2+- and Sr2+-activated tensions were measured in functionally skinned fibres from the rabbit diaphragm. The fibres could be classified into two types on the basis of activation by divalent cations. Type S fibres required a slightly higher [Ca2+] than [Sr2+] for half-maximal activation of tension. Type F fibres required 2 times higher [Ca2+] than type S and 8.5 times higher [Sr2+]. Sodium dodecyl sulphate disc gels of type F fibres showed protein band patterns similar to those of fast-twitch muscle; those of type S fibres were identical to those of slow-twitch fibres. Sodium dodecyl sulphate disk gels of mixed, non-classified diaphragm fibres and an actual count of randomly selected fibres classified by Ca2+/Sr2+ tension characteristics showed the diaphragm to be composed of 60% fast-twitch fibres and 40% slow-twitch fibres. The protein stoichiometries of the two fibre types are consonant with the accepted stoichiometries for skeletal muscle. The percentages of type F and type S fibres in the diaphragm suggest that high adenosine-triphosphatase activity shown by histochemical techniques (Davies & Gunn, 1972) is associated with the type F fibres, and low activity with the type S fibres.

Original languageEnglish (US)
Pages (from-to)345-352
Number of pages8
JournalThe Journal of Physiology
Volume295
Issue number1
DOIs
StatePublished - Oct 1 1979

ASJC Scopus subject areas

  • Physiology

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