Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection

M. I. Bukrinsky, T. L. Stanwick, M. P. Dempsey, Mario Stevenson

Research output: Contribution to journalArticle

451 Citations (Scopus)

Abstract

To better understand the basis for human immunodeficiency virus type 1 (HIV-1) persistence and latency, the form in which viral DNA exists in the peripheral T lymphocyte reservoir of infected individuals was investigated. In asymptomatic individuals, HIV-1 was harbored predominantly as full-length, unintegrated complementary DNA. These extrachromosomal DNA forms retained the ability to integrate upon T cell activation in vitro. In patients with acquired immunodeficiency syndrome (AIDS), there was an increase in integrated relative to extrachromosomal DNA forms. By analysis of DNA from patient lymphocyte subpopulations depleted of human lymphocyte antigen-Dr receptor-positive cells, quiescent T cells were identified as the source of extrachromosomal HIV-1 DNA. Thus quiescent T lymphocytes may be a major and inducible HIV-1 reservoir in infected individuals.

Original languageEnglish
Pages (from-to)423-427
Number of pages5
JournalScience
Volume254
Issue number5030
StatePublished - Oct 18 1991
Externally publishedYes

Fingerprint

Virus Diseases
HIV-1
Viruses
T-Lymphocytes
DNA
Antigen Receptors
Lymphocyte Subsets
Viral DNA
Acquired Immunodeficiency Syndrome
Complementary DNA
Lymphocytes

ASJC Scopus subject areas

  • General

Cite this

Bukrinsky, M. I., Stanwick, T. L., Dempsey, M. P., & Stevenson, M. (1991). Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection. Science, 254(5030), 423-427.

Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection. / Bukrinsky, M. I.; Stanwick, T. L.; Dempsey, M. P.; Stevenson, Mario.

In: Science, Vol. 254, No. 5030, 18.10.1991, p. 423-427.

Research output: Contribution to journalArticle

Bukrinsky, MI, Stanwick, TL, Dempsey, MP & Stevenson, M 1991, 'Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection', Science, vol. 254, no. 5030, pp. 423-427.
Bukrinsky MI, Stanwick TL, Dempsey MP, Stevenson M. Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection. Science. 1991 Oct 18;254(5030):423-427.
Bukrinsky, M. I. ; Stanwick, T. L. ; Dempsey, M. P. ; Stevenson, Mario. / Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection. In: Science. 1991 ; Vol. 254, No. 5030. pp. 423-427.
@article{ddb4b9f8b12f436daf639cb045fec355,
title = "Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection",
abstract = "To better understand the basis for human immunodeficiency virus type 1 (HIV-1) persistence and latency, the form in which viral DNA exists in the peripheral T lymphocyte reservoir of infected individuals was investigated. In asymptomatic individuals, HIV-1 was harbored predominantly as full-length, unintegrated complementary DNA. These extrachromosomal DNA forms retained the ability to integrate upon T cell activation in vitro. In patients with acquired immunodeficiency syndrome (AIDS), there was an increase in integrated relative to extrachromosomal DNA forms. By analysis of DNA from patient lymphocyte subpopulations depleted of human lymphocyte antigen-Dr receptor-positive cells, quiescent T cells were identified as the source of extrachromosomal HIV-1 DNA. Thus quiescent T lymphocytes may be a major and inducible HIV-1 reservoir in infected individuals.",
author = "Bukrinsky, {M. I.} and Stanwick, {T. L.} and Dempsey, {M. P.} and Mario Stevenson",
year = "1991",
month = "10",
day = "18",
language = "English",
volume = "254",
pages = "423--427",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5030",

}

TY - JOUR

T1 - Quiescent T lymphocytes as an inducible virus reservoir in HIV-1 infection

AU - Bukrinsky, M. I.

AU - Stanwick, T. L.

AU - Dempsey, M. P.

AU - Stevenson, Mario

PY - 1991/10/18

Y1 - 1991/10/18

N2 - To better understand the basis for human immunodeficiency virus type 1 (HIV-1) persistence and latency, the form in which viral DNA exists in the peripheral T lymphocyte reservoir of infected individuals was investigated. In asymptomatic individuals, HIV-1 was harbored predominantly as full-length, unintegrated complementary DNA. These extrachromosomal DNA forms retained the ability to integrate upon T cell activation in vitro. In patients with acquired immunodeficiency syndrome (AIDS), there was an increase in integrated relative to extrachromosomal DNA forms. By analysis of DNA from patient lymphocyte subpopulations depleted of human lymphocyte antigen-Dr receptor-positive cells, quiescent T cells were identified as the source of extrachromosomal HIV-1 DNA. Thus quiescent T lymphocytes may be a major and inducible HIV-1 reservoir in infected individuals.

AB - To better understand the basis for human immunodeficiency virus type 1 (HIV-1) persistence and latency, the form in which viral DNA exists in the peripheral T lymphocyte reservoir of infected individuals was investigated. In asymptomatic individuals, HIV-1 was harbored predominantly as full-length, unintegrated complementary DNA. These extrachromosomal DNA forms retained the ability to integrate upon T cell activation in vitro. In patients with acquired immunodeficiency syndrome (AIDS), there was an increase in integrated relative to extrachromosomal DNA forms. By analysis of DNA from patient lymphocyte subpopulations depleted of human lymphocyte antigen-Dr receptor-positive cells, quiescent T cells were identified as the source of extrachromosomal HIV-1 DNA. Thus quiescent T lymphocytes may be a major and inducible HIV-1 reservoir in infected individuals.

UR - http://www.scopus.com/inward/record.url?scp=0026054892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026054892&partnerID=8YFLogxK

M3 - Article

C2 - 1925601

AN - SCOPUS:0026054892

VL - 254

SP - 423

EP - 427

JO - Science

JF - Science

SN - 0036-8075

IS - 5030

ER -