Quercetin Protects Against Linoleic Acid-Induced Porcine Endothelial Cell Dysfunction

Gudrun Reiterer, Michal J Toborek, Bernhard Hennig

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Consumption of plant phenolics, such as quercetin, may be associated with decreased risk of cardiovascular disease by stabilizing and protecting vascular endothelial cells against oxidative and proinflammatory insults. The present study focused on the effect of quercetin on linoleic acid-induced oxidative stress and the inflammatory pathways of nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). Because the transcription factor peroxisome proliferator activated receptor γ(PPARγ) was reported to downregulate inflammatory pathways, we further investigated the effect of quercetin on PPARγ. Porcine pulmonary-arterial endothelial cells were activated with linoleic acid in the presence or absence of quercetin. Oxidative stress was markedly induced by endothelial cell exposure to linoleic acid and diminished by treatment with quercetin as measured via the oxidation of 2′,7′-dichlorofluorescin. Quercetin reduced linoleic acid-mediated binding activity of NF-κB and AP-1 and mRNA levels of inflammatory genes such as interleukin-6 (IL-6) and vascular cell adhesion molecule-1 (VCAM-1). Cotreatment of linoleic acid plus quercetin or vitamin E also decreased linoleic acid-induced binding activity of PPARγ. These data suggest that quercetin has potent antioxidative and anti-inflammatory properties and protects endothelial cells against linoleic acid-mediated cell dysfunction.

Original languageEnglish
Pages (from-to)771-775
Number of pages5
JournalJournal of Nutrition
Volume134
Issue number4
StatePublished - Apr 1 2004
Externally publishedYes

Fingerprint

Quercetin
Linoleic Acid
quercetin
endothelial cells
linoleic acid
Swine
Endothelial Cells
swine
Peroxisome Proliferator-Activated Receptors
Transcription Factor AP-1
blood vessels
Oxidative Stress
oxidative stress
Vascular Cell Adhesion Molecule-1
risk reduction
anti-inflammatory activity
Vitamin E
cell adhesion
interleukin-6
cardiovascular diseases

Keywords

  • Atherosclerosis
  • Fatty acids
  • PPAR
  • Quercetin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Quercetin Protects Against Linoleic Acid-Induced Porcine Endothelial Cell Dysfunction. / Reiterer, Gudrun; Toborek, Michal J; Hennig, Bernhard.

In: Journal of Nutrition, Vol. 134, No. 4, 01.04.2004, p. 771-775.

Research output: Contribution to journalArticle

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